丁香有效部位对LDL中脂类、蛋白氧化修饰及光谱学变化的抑制

LDL氧化修饰是致动脉粥样硬化的关键性因素。其中,在氧化修饰过程中LDL所含脂类、蛋白及其光谱学性质均会受到影响。前期研究结果发现丁香对LDL氧化修饰具有很强的抑制作用。但是,丁香是否是通过抑制脂类及蛋白氧化来实现,以及丁香是否能抑制LDL氧化修饰后光谱学性质的改变,这些问题目前均不清楚。据此,运用紫外吸收光谱、荧光光谱等方法研究了丁香有效部位(effective fraction from clove,EFC)对LDL氧化修饰的抑制效果。研究结果表明,EFC能有效延缓脂质氧化过程中共轭二烯(CD)增殖及推后其达到最大值,并抑制胆固醇降解;也可抑制蛋白组分apoB100中Trp荧光猝灭及Lys氧化修饰和荧光光谱变化;同时也能抑制LDL中脂质与蛋白过氧化产物-脂褐素的生成;此外,该部位对LDL 氧化修饰过程中紫外-可见光谱学特征的改变也有影响。论文将为丁香抗AS功能食品研发提供参考。     

High Hydrostatic Pressure Induces a Lipid Phase Transition and Molecular Rearrangements in Low‐Density Lipoprotein Nanoparticles

Low‐density lipoproteins (LDL) are natural lipid transporter in human plasma whose chemically modified forms contribute to the progression of atherosclerosis and cardiovascular diseases accounting for a vast majority of deaths in westernized civilizations. For the development of new treatment strategies, it is important to have a detailed picture of LDL nanoparticles on a molecular basis. Through the combination of X‐ray and neutron small‐angle scattering (SAS) techniques with high hydrostatic pressure (HHP) this study describes structural features of normolipidemic, triglyceride‐rich and oxidized forms of LDL. Due to the different scattering contrasts for X‐rays and neutrons, information on the effects of HHP on the internal structure determined by lipid rearrangements and changes in particle shape becomes accessible. Independent pressure and temperature variations provoke a phase transition in the lipid core domain. With increasing pressure an interrelated anisotropic deformation and flattening of the particle are induced. All LDL nanoparticles maintain their structural integrity even at 3000 bar and show a reversible response toward pressure variations. The present work depicts the complementarity of pressure and temperature as independent thermodynamic parameters and introduces HHP as a tool to study molecular assembling and interaction processes in distinct lipoprotein particles in a nondestructive manner.     

On the Geometry of Darboux Transformations for the KP Hierarchy and its Connection with the Discrete KP Hierarchy

We tackle the problem of interpreting the Darboux transformation for the KP hierarchy and its relations with the modified KP hierarchy from a geometric point of view. This is achieved by introducing the concept of a Darboux covering. We construct a Darboux covering of the KP equations and obtain a new hierarchy of equations, which we call the Darboux-KP hierarchy (DKP). We employ the DKP equations to discuss the relationships among the KP equations, the modified KP equations, and the discrete KP equations. Our approach also handles the various reductions of the KP hierarchy. We show that the KP hierarchy is a projection of the DKP, the mKP hierarchy is a DKP restriction to a suitable invariant submanifold, and that the discrete KP equations are obtained as iterations of the DKP ones. Received: 23 July 1996 / Accepted: 6 January 1997     

On the modified discrete KP equation with self-consistent sources

The modified discrete KP equation is the Bäcklund transformation for the Hirota’s discrete KP equation or the Hirota-Miwa equation. We construct the modified discrete KP equation with self-consistent sources via source generation procedure and clarify the algebraic structure of the resulting coupled modified discrete KP system by presenting its discrete Gram-type determinant solutions. It is also shown that the commutativity between the source generation procedure and Bäcklund transformation is valid for the discrete KP equation. Finally, we demonstrate that the modified discrete KP equation with self-consistent sources yields the modified differential-difference KP equation with self-consistent sources through a continuum limit. The continuum limit of an explicit solution to the modified discrete KP equation with self-consistent sources also gives the explicit solution for the modified differential-difference KP equation with self-consistent sources.     

Structural and Nonlinear Optical Characteristics of In Vitro Glycation of Human Low-Density Lipoprotein,as a Function of Time

Modified low-density lipoprotein (LDL) is a well-known risk marker for diabetes and cardiovascular disease. In vitro and in vivo studies have shown that native LDL particles, when modified by oxidation and/or glycation processes, become proatherogenic. Other studies have shown that high LDL concentrations also contribute to atherogenic diseases. In the present in vitro study, we investigate structural characteristics, linear and nonlinear optical properties of LDL particles modified by glycation, compared to modified-control and non-modified LDL (LDL_nat). LDL particles were isolated from normolipidemic individuals and aliquots were incubated in PBS (LDL_control) and glycated (LDL_glyc) with glycolaldehyde (GAD) from 2 to 6 days. The nonlinear optical Z-Scan experiments indicate that GAD modifies the optical properties of the LDL. These results indicate the application of a nonlinear optical technique as a tool to investigate the characteristics of LDL particles, in particular when modifications are induced in the particles by glycation.     

Work function changes and surface chemistry of oxygen, hydrogen, and carbon on indium tin oxide

The surface chemistry of indium tin oxide (ITO) has been investigated with Auger electron spectroscopy (AES) and high resolution electron energy loss spectroscopy (HREELS). A vibrating Kelvin probe (KP) with a graphite reference was used to monitor the absolute work function (Φ) of ITO as a function of chemical modification. The ITO was exposed in situ to molecular hydrogen (H₂), hot-filament-activated oxygen (O₂^*), and hot-filament-activated deuterium (D₂^*). The initial Φ of ITO was determined to be 5.2 eV, and surface chemical changes had strong effects on this value, as seen by KP. Exposure of clean ITO to O₂^* increased Φ to 5.6 eV, but the increase was short-lived. The changes in Φ over time were correlated with the uptake of carbon impurities in ultra high vacuum (UHV), as monitored by AES.

The HREELS of ITO revealed significant hydrocarbon impurities. Chemical reduction of ITO produced a metallic surface and dehydrogenated the adsorbed hydrocarbons. Both re-oxidation of metallic ITO and oxidation of clean ITO temporarily removed adventitious carbon from the surface, but oxidized ITO adsorbed an even larger quantity of carbon over time.     

Self-Consistent Sources Extensions of Modified Differential-Difference KP Equation

In this paper, we investigate a modified differential-difference KP equation which is shown to have a continuum limit into the mKP equation. It is also shown that the solution of the modified differential-difference KP equation is related to the solution of the differential-difference KP equation through a Miura transformation. We first present the Grammian solution to the modified differential-difference KP equation, and then produce a coupled modified differential-difference KP system by applying the source generation procedure. The explicit N-soliton solution of the resulting coupled modified differential-difference system is expressed in compact forms by using the Grammian determinant and Casorati determinant. We also construct and solve another form of the self-consistent sources extension of the modified differential-difference KP equation, which constitutes a Bäcklund transformation for the differential-difference KP equation with self-consistent sources.     

Some quasi-periodic solutions to the Kadometsev-Petviashvili and modified Kadometsev-Petviashvili equations

The Kadometsev-Petviashvili (KP) and modified KP (mKP) equations are retrieved from the first two soliton equations of coupled Korteweg-de Vries (cKdV) hierarchy. Based on the nonlinearization of Lax pairs, the KP and mKP equations are ultimately reduced to integrable finite-dimensional Hamiltonian systems in view of the r-matrix theory. Finally, the resulting Hamiltonian flows are linearized in Abel-Jacobi coordinates, such that some specially explicit quasi-periodic solutions to the KP and mKP equations are synchronously given in terms of theta functions through the Jacobi inversion.     

In vitro studies of PBT Nonwoven Fabrics adsorbent for the removal of low density lipoprotein from hyperlipemia plasma

Polyanion ligands such as acrylic acid (AA) and heparin were grafted on PBT Nonwoven Fabrics (PBTNF) to study their effect on the adsorption of low density lipoprotein (LDL). These modified PBTNFs were characterized by Horizontal Attenuated Total Reflectance Fourier Transform Infrared spectroscopy and X-ray Photoelectron spectroscopy. The blood compatibilities of the modified PBTNFs were examined using in vitro hemolysis rate (HR), platelet adhesion, total protein (TP) and activated partial thromboplastin time. The results showed that direct immobilized heparin could improve PBTNF-PAA's blood compatibility and decrease the adsorption capability of useful high density lipoprotein, but would possess so low bioactivity that could not further improve the absorption of LDL and TC. Since the PBTNF-PAA55-Heparin adsorbent had quite good adsorption selectivity for these proteins, it can be an excellent candidate for depletion of LDL with good blood compatibility.     

Three types of generalized Kadomtsev－Petviashvili equations arising from baroclinic potential vorticity equation

By means of the reductive perturbation method, three types of generalized (2+1)-dimensional Kadomtsev--Petviashvili (KP) equations are derived from the baroclinic potential vorticity (BPV) equation, including the modified KP (mKP) equation, standard KP equation and cylindrical KP (cKP) equation. Then some solutions of generalized cKP and KP equations with certain conditions are given directly and a relationship between the generalized mKP equation and the mKP equation is established by the symmetry group direct method proposed by Lou et al. From the relationship and the solutions of the mKP equation, some solutions of the generalized mKP equation can be obtained. Furthermore, some approximate solutions of the baroclinic potential vorticity equation are derived from three types of generalized KP equations.     

Effects of dust size distribution on nonlinear waves in a dusty plasma

For two-dimensional unmagnetized dusty plasmas with many different dust grain species, a Kadomtsev--Petviashvili (KP) equation, a modified KP (mKP) equation and a coupled KP(cKP) equation for small, but finite amplitude dust-acoustic solitary waves are obtained for different physical conditions respectively. The influence of an arbitrary dust size distribution described by a polynomial expressed function on the properties of dust-acoustic solitary waves is investigated numerically. How dust size distribution affects the sign and the magnitude of nonlinear coefficient A(D) of KP (mKP) equation is also discussed in detail. It is noted that whether a compressive or a rarefactive solitary wave exists depends on the dust size distribution in some dusty plasmas.     

Infinitely-many conservation laws for two (2+1)-dimensional nonlinear evolution equations in fluids

In this paper, a method that can be used to construct the infinitely-many conservation laws with the Lax pair is generalized from the (1+1)-dimensional nonlinear evolution equations (NLEEs) to the (2+1)-dimensional ones. Besides, we apply that method to the Kadomtsev–Petviashvili (KP) and Davey–Stewartson equations in fluids, and respectively obtain their infinitely-many conservation laws with symbolic computation. Based on that method, we can also construct the infinitely-many conservation laws for other multidimensional NLEEs possessing the Lax pairs, including the cylindrical KP, modified KP and (2+1)-dimensional Gardner equations, in fluids, plasmas, optical fibres and Bose–Einstein condensates.     

Miura and auto-Backlund transformations for the q-deformed KP and q-deformed modified KP hierarchies

The Miura and anti-Miura transformations between the q-deformed KP and the q-deformed modified KP hierarchies are investigated in this paper. Then the auto-Backlund transformations for the q-deformed KP and q-deformed modified KP hierarchies are constructed through the combinations of the Miura and anti-Miura transformations. And the corresponding results are also generalized to the constrained cases. At last, some examples of Miura and auto-Backlund transformations are given.     

光纤与生物分子界面上固定FITC标记抗体的荧光光谱

采用化学方法把FITC标记羊抗人抗体IgG共价固定到三氨基三乙氧基硅烷和戊二醛(APTES-Glu)修饰的石英光纤纤芯表面。通过研究未经过任何修饰的纤芯表面的吸附以及共价固定的FITC标记羊抗人抗体的荧光光谱性质发现:共价结合到纤芯表面的FITC荧光光谱相对于溶液中FITC的荧光峰位红移约9nm,而物理吸附的FITC的荧光峰位移动约4nm,而且两者相对荧光强度相差6倍。而在固定的人血清蛋白进行免疫反应后,FITC的荧光峰位只移动3～4nm,除此之外,研究了抗体共价键固定的稳定性问题。结果表明:共价键结合抗体的数量要大于表面吸附,共价键固定的FITC标记羊抗人抗体与光纤表面间的相互作用较吸附于光纤表面的FITC标记羊抗人抗体间的相互作用更强。这对于建立一种通过荧光光谱识别固体表面与生物分子的吸附还是共价结合的判据提供了物理基础。     

Virasoro symmetry of constrained KP hierarchies

The conventional formulation of additional nonisospectral sysmmetries for the full Kadomtsev-Petviashvili (KP) integrable hierarchy is not compatible with the reduction to the important class of constrained KP (cKP) integrable models. This paper solves explicitly the problem of compatibility of the Virasoro part of additional symmetries with the underlying constraints of cKP hierarchies. Our construction involves an appropriate modification of the standard additional-symmetry flows by adding a set of “ghost symmetry” flows. We also discuss the special case of cKP — truncated KP hierarchies, obtained as Darboux-Bäcklund orbits of initial purely differential Lax operators. Our construction establishes the condition for commutativity of the additional-symmetry flows with the discrete Darboux-Bäcklund transformations of cKP hierarchies leading to a new derivation of the string-equation constraint in matrix models.     

RECURSION OPERATORS FOR KP,mKP AND HARRY DYM HIERARCHIES

In this paper, we give a unified construction of the recursion operators from the Lax representation for three integrable hierarchies: Kadomtsev–Petviashvili (KP), modified Kadomtsev–Petviashvili (mKP) and Harry Dym under n-reduction. This shows a new inherent relationship between them. To illustrate our construction, the recursion operator are calculated explicitly for 2-reduction and 3-reduction.     

B?cklund transformations,consistent Riccati expansion solvability,and soliton–cnoidal interaction wave solutions of Kadomtsev–Petviashvili equation

The famous Kadomtsev-Petviashvili(KP)equation 1 s a classical equation In soliton tneory.A Backlund transformation between the KP equation and the Schwarzian KP equation is demonstrated by means of the truncated Painlev6 expansion in this paper.One-parameter group transformations and one-parameter subgroup-invariant solutions for the extended KP equation are obtained.The consistent Riccati expansion(CRE) solvability of the KP equation is proved.Some interaction structures between soliton-cnoidal waves are obtained by CRE and several evolution graphs and density graphs are plotted.     

Graded Symmetry Algebras of Time-Dependent Evolution Equations and Application to the Modified KP Equations

Abstract

By starting from known graded Lie algebras, including Virasoro algebras, new kinds of time-dependent evolution equations are found possessing graded symmetry algebras. The modified KP equations are taken as an illustrative example: new modified KP equations with m arbitrary time-dependent coefficients are obtained possessing symmetries involving m arbitrary functions of time. A particular graded symmetry algebra for the modified KP equations is derived in this connection homomorphic to the Virasoro algebras.

Dedicated to Prof. W.I. Fushchych on the occasion of his 60th birthday     

EPR investigation of cell membrane fluidity upon external oxidative stimulus

The perturbation of the physical state of cell membranes triggered by an external oxidative stimulus has been studied with sperm cells which were chosen as a model system. Electron paramagnetic resonance (EPR) spectroscopy was applied and three different nitroxides bearing a paramagnetic group on the 5th, 7th and 13th carbon of the stearic acid acyl chain were used to probe different regions of the membrane. The theoretical simulations of the experimental spectra indicate distinct domains in the intact cell plasma membrane where local steric constraints impose different degrees of motional averaging experienced by the reporter group. Upon the external oxidative stimulus the spectral changes were proportional to the accumulated oxidation products and were detected only for the reporter group residing close to the lipid-water interface. The EPR spectra reveal that the motion of the reporter group has slowed down in the oxidized cells and that the oxidatively modified membrane shows up as a more rigid structure as compared with the native state. The results also indicate that the oxidation-induced spectral changes are supressed in the presence of gangliosides.