共查询到20条相似文献,搜索用时 15 毫秒
1.
二茂铁标记DNA电化学探针的研制及性质研究 总被引:9,自引:0,他引:9
以乙基-(3-二甲基丙基)碳化二亚胺盐酸(EDC)为偶联活化剂,利用缩合反应分别将电化学活性物质氨基二茂铁(Aminoferrocene,AFC)和苯基二茂铁(Ferrocencearboxadehyde,FCA)成功地标记在变性小牛胸腺DNBA片段上,制备成二茂铁标记DNA探针,分别采用电化学,紫外,红外光谱等方法研究了二茂铁标记DAN探针的性质,计算了二茂铁的标记效率,变性小牛胸腺DNA的反应效率以及二茂铁化合物与变性小牛胸腺DNA的反应比率,实验表明,氨基二茂铁和醛基二茂铁与DNA上的磷酸基是以1:1比率进行的反应,该标记反应不影响DNA链碱基的紫外吸收,二茂铁标记DNA探针在石墨电极上有良好的电化学影响,将制备好的二茂铁标记DNA电化学探置于冰箱中冷冻(温度低于-15度)保存3个月后用于测定,峰电流仅下降1.2%。 相似文献
2.
ZHAO Bing 《高等学校化学研究》1995,(2)
The interaction of trivalent lanthanide ions and divalent calcium ions with sph-'ngomyelm bilayer has been studied by FT-Raman spectroscopy.The results showed that the bonding of metal ions to the phosphate group of sphingomyelin bi-iayer,either La3 or Ca2 did not change the conformation of the choline group,that is,O-C-C-N is still in its gauche conformation.The presence of metal ions changed the states of the interfacial region from liquid-like to amorphous state and even to crystalline.They increased the fluidity of acyl chains of sphingomyelin bilaver and made them packed disorderly. 相似文献
3.
用密度泛函理论方法BLYP/6-311++G(2df,2pd)对尿素分子的平衡几何构型进行了优化,并计算了该分子的谐力场.使用Wilson的GF矩阵方法,对尿素分子的振动基频进行了理论研究.根据振动频率的势能分布对此分子的振动基频进行了理论归属,计算的振动频率和能级指认均同实验结果吻合较好. 相似文献
4.
Alberto Federman Neto Joseph Miller Vnia Faria de Andrade Susana Yumi Fujimoto Mrcia Maísa de Freitas Afonso Fernando Costa Archanjo Vítor Andr Darin Mrcio Luís Andrade e Silva urea Donizete Lanchote Borges Gino Del Ponte 《无机化学与普通化学杂志》2002,628(1):209-216
An improved technique for the preparation of ferrocenecarboxaldehyde and two new methods for the synthesis of ferrocenemonocarboxylic acid from the aldehyde or from acetyl ferrocene are described. Using the aldehyde or the monocarboxylic acid as starting materials, some important ferrocene key intermediates were prepared: monochlorocarbonyl ferrocene, alkali metal and ammonium ferrocenoates, ferrocene carboxyaldehyde diethylacetal, ferrocenecarboxamide, et …, as well as a new ferrocene derivative: piperazinium diferrocenoate. 相似文献
5.
6.
采用密度泛函理论DFT(B3LYP)方法,以6-31G*为基组对ClC(O)NCS的反式和顺式两种构型的几何结构、振动谐性力场和红外光谱进行了研究.B3LYP/6-31G*的理论力场由适用于B3LYP/6-31G*计算水平和大多数有机分子的一套固定标度因子进行标度.根据标度后的理论力场进行简正坐标分析得到的势能分布(PED)和红外光谱强度值对ClC(O)NCS分子的顺式和反式两种构型的振动基频进行了理论归属. 相似文献
7.
8.
Byung Joon Lim Inhong Hwang Andrew D. Ellington Jonathan L. Sessler 《Helvetica chimica acta》2019,102(2)
A series of ferrocene derivatives, which have diverse redox potentials modulated by functional groups, have been synthesized as potential ‘multi‐potential’ probes. A Hammett constant analysis revealed a linear free energy correlation between the redox potentials and the electron density of the ferrocene derivatives as determined by the choice of functional group used to modify the ferrocene core. 相似文献
9.
10.
香豆素酮染料光谱性质的研究 总被引:1,自引:0,他引:1
近年来,许多具有“推一拉”电子结构的分子内电行转移激光染料的光谱性质的研究引起人们极大的关注[1-5].研究染料在不同条件下光物理性质行为不仅具有重要的理论意义,而且使激光染料能有效地应用于光功能材料.香豆素酮染料作为一种重要的激光染料,已经在光聚合、光固化等领域中得到广泛应用[6,7].在这类化合物中不同位置的取代对其光物理性质的研究已有报导[8,9],但香豆素酮染料在不同的非质子传递溶剂中的光物理行为的研究至今未见报导.鉴于以前关于香豆素染料文献报导常涉及到用醇、水等质子传递溶剂,为了避免溶剂与溶质间的氢… 相似文献
11.
利用IR,EPR和UV-VIS方法对系列取代戊二烯基钛,钒羰基化合物进行了表征,用EHMO方法对开环夹心化合物[2,4-(CH_3)_2C_2H_5]_2VCO和开、闭环夹心化合物[2,4(CH_3)_2C_5H_5](C_5H)5)VCO进行了计算,对开环夹心羰基化合物,开、闭环夹心羰基化合物及闭环夹心羰基化合物进行了比较.结果表明,开、闭环夹心羰基化合物的性质介于开环夹心羰基化合物和闭环夹心羰基化合物之间,且更接近于开环夹心羰基化合物,开环配体与Ti,V间的化学键较强,对于化合物性质影响较大. 相似文献
12.
本文用自旋捕捉-ESR技术研究了二茂戊铁、环二烯和胺甲基类取代二茂铁的紫外光解过程中的活泼自由基。结果表明,二茂铁光解初级过程中生成的自由基为(C_5H_5Fe)·和C_5H_5·,其衍生物光解则生成(C_5H_5Fe)·和RC_5H_4·,而(C_5H_5Fe)·还可以进一步分解给出C_5H_5和Fe。 相似文献
13.
《Electroanalysis》2003,15(13):1139-1142
Electrochemical properties of Fc‐PEM films have been studied by changing the chemical structure of the polymer chains and the content of Fc moiety in the film systematically. We have prepared a series of PEM films by a layer‐by‐layer deposition of polycations, Fc‐modified poly(allylamine) (Fc‐PAA) and poly(ethyleneimine) (Fc‐PEI), and polyanionic poly(vinyl sulfate) (PVS) on the surface of a gold electrode. The redox properties of the Fc‐PAA/PVS and Fc‐PEI/PVS films depended significantly on the content of Fc moiety in the polymer chains and on the polymer type. Fc‐ PAA and Fc‐PEI polymer chains can penetrate 3 or 4 PAA/PVS bilayers inserted between the redox polymers and electrode. The Fc‐PAA film‐modified electrode can be used for electrocatalytic oxidation of ascorbic acid. 相似文献
14.
15.
4,4′-二(硬脂酰胺基)-二苯甲烷(BSAPM)在LiClO4/碳酸丙烯酯(PC)中能形成超分子有机凝胶。用循环伏安法研究了包埋在凝胶中的二茂铁的氧化还原行为。结果表明,有机凝胶内的二茂铁仍具有氧化还原活性,其氧化还原行为是受扩散控制的单电子可逆转移过程。与溶液相比,最低化凝胶浓度下凝胶中二茂铁和二茂铁离子的扩散系数分别从5.62×10-6cm2/s和6.47×10-6cm2/s下降为3.32×10-6cm2/s和4.41×10-6cm2/s,且随凝胶因子浓度的增加,凝胶中二茂铁和二茂铁离子的扩散系数降低。 相似文献
16.
对6个新的烷氧基亚甲基二苯胂基二茂铁衍生物进行质谱分析研究,在它们的电子轰击电离质谱中显现了强的分子离子峰和表征主体结构的一系列特征峰。通过磁场/静电场联动扫描和精确质量的测定探索了这类化合物的质谱裂解规律。 相似文献
17.
二茂铁酰基衍生物的研究进展 总被引:15,自引:2,他引:15
综述了近年来二茂铁酰基衍生物如二茂铁甲醛、二茂铁酮和二茂铁甲酸的合成及反应的研究进展,简要介绍了它在生物学、医学和电化学等领域的应用。参考文献73篇。 相似文献
18.
Michaele J. Hardie 《Supramolecular chemistry》2013,25(1):7-10
Combinations of ferrocene with unsubstituted calix [n]arenes, n =4 and 5, in toluene afford crystalline inclusion complexes (calix[4]arene) 3 (ferrocene) [hexagonal, a =14.7797(4), c =18.4400(4) Å, P6M3/m , ρ = 1.389 g.cm ?1 , Z =2, R 1 =0.0963 (on 1401 observed data [ I >2 σ ( I )])] as a lattice-type inclusion complex, and (calix[5]arene)(toluene) 0.55 (ferrocene) 0.45 [monoclinic, a =9.6908(8), b =15.069(1), c =22.957(1) Å, β = 90.155(5)°, P21/m , ρ =1.318 g.cm ?1 , Z =4, R 1 =0.1005 (on 3130 observed data [ I >2 σ ( I )])], that shows endo -cavity complexation of ferrocene by the calixarene. 相似文献
19.
Structural and optoelectronic properties of ferrocene (FeC10H10) using various exchange correlation potentials including Spin Polarized Generalized Gradient Approximation (SPGGA), Hybrid Density Functional Theory (SPHYB‐DFT), and hybrid density functional Becke3LYP are investigated. Obtained bandgap by the SPHYB‐DFT and SPGGA methods show consistency with the experiment, that are indirect and direct, respectively. The cell size effects on physical properties of ferrocene studied about two types of its lattice parameters ( I and II ). The calculated results reveal that the cell size and the lattice parameters have a remarkable effect on optoelectronic and magnetic properties of ferrocene. However, there is no significant difference between I and II within molecular, structural and charge transitions in calculating UV/Vis spectrum. The calculated electronic absorption spectrum is in good agreement with experiment, in which two major electron‐transition bands derived from d–d (n → n*) and n → π* metal to ligand. NBO analyses show that there are strong donor‐acceptor interactions between central Fe atoms and cyclopentadienyl (Cp) rings that these results are in close agreement with contour plots of charge densities for prediction of the strong covalent bond between C and Fe. The optoelectronic properties of ferrocene predict that it can be efficiently used in the semiconductor devices. 相似文献
20.
StructuresandVibrationalSpectraofC2andLaC+2Clusters*WUZhi-jian,MENGQing-boandZHANGSi-yuan**(ChangchunInstituteofAppliedChemis... 相似文献