秋水仙碱与人血清白蛋白相互作用的光谱学和电化学研究

用荧光光谱、紫外吸收光谱和电化学方法研究了在近似生理条件下秋水仙碱与人血清白蛋白的相互作用.研究表明,秋水仙碱对人血清白蛋白的荧光猝灭主要是静态猝灭过程,秋水仙碱使人血清白蛋白的构象发生变化.与此同时,在pH=7.4、含0.05%吐温-20的磷酸盐缓冲液中,秋水仙碱在玻碳电极上出现一不可逆的氧化峰,加入人血清白蛋白后秋水仙碱的氧化峰电位正移,峰电流下降.此外,利用光谱学方法和电化学方法测定的秋水仙碱与人血清白蛋白相互作用的结合常数和结合位点数吻合.     

苯乙烯嘧啶化合物的合成及其作为pH探针的光谱特点

合成并表征了化合物4,6-二[（4′-二乙基胺基）苯乙烯基]嘧啶-2-醇。 通过研究该化合物在不同pH值的磷酸盐缓冲溶液中紫外-可见吸收光谱和荧光光谱的变化,发现λmax约为500 nm的吸收峰在溶液pH=6.78中吸收强度最大。 荧光发射峰随着pH值的升高逐渐减弱,直至淬灭（pH=5.51）。     

秋水仙碱与醇脱氢酶相互作用的光谱学和分子对接研究

在模拟人体生理条件下,利用光谱学和分子对接技术研究了秋水仙碱(Col)与醇脱氢酶(ADH)之间的相互作用机理。不同温度条件下的荧光光谱实验证明秋水仙碱能够通过氢键和范德华力与ADH结合形成复合物,从而抑制ADH的酶活力并引起ADH荧光的静态淬灭;不同温度条件下,Hill系数nH均约等于1,表明秋水仙碱对后继配体没有协同作用;同步荧光和红外光谱实验表明秋水仙碱对ADH的构象没有影响。紫外光谱和分子对接研究表明Col与乙醇类似,都是通过O上的孤对电子与ADH活性中心的Zn离子结合并进行定位。     

应用荧光光度法研究加替沙星与胃蛋白酶的相互作用

应用荧光光度法研究了加替沙星(GTFX)在pH 2.0的氯化钾-盐酸介质中,与胃蛋白酶(PS)的相互作用(结合作用)。讨论了GTFX对PS内源荧光的淬灭机理,证实了GTFX对PS的荧光淬灭属于静态猝灭并测得了在288K,298K及310K3种温度下GTFX与PS间的猝灭常数(KSV)和结合常数(K),以及GTFX与PS间结合位点数(n)。     

家蝇幼虫抗菌肽MDL-1的荧光特性分析

本文研究了家蝇幼虫抗菌肽MDL-1的荧光光谱和淬灭剂对内源性荧光的影响。家蝇幼虫抗菌肽MDL-1在激发波长280 nm时,其荧光光谱为酪氨酸（Tyr）残基和色氨酸（Trp）残基共同提供。结果表明,KI不能淬灭抗菌肽MDL-1的Trp残基的荧光,而丙烯酰胺（Acr）能淬灭几乎所有的Trp残基的荧光（f-0．92）;这说明,Trp残基不是位于抗菌肽分子的表面,而是位于分子的内部。     

金属离子协同效应对血红蛋白分子荧光光谱的影响

本文采用荧光光谱法对血红蛋白(Hb)与Zn^2 、Cu^2 、Ag^ 三种离子的单一离子及混合离子的相互作用进行了研究。结果表明,Zn^2 对Hb有较强的荧光增强作用,Cu^2 具有较强的荧光淬灭作用,而Ag^ 有较弱的荧光淬灭作用;在二元金属离子与Hb的混合体系中,由于金属离子与Hb之间存在竞争配位作用或离子之间相互制约及协同效应,使混合体系中Hb的荧光光谱变化较单一金属离子有明显的差异。     

胰岛素分子存在形态及结构变化的同步荧光光谱研究

利用同步荧光光谱技术，在波长差Δλ为30nm和80nm下对胰岛素的同步荧光光谱特征进行研究，发现胰岛素中的酪氨酸和色氨酸残基的同步荧光光谱随浓度改变而发生变化，该变化与胰岛素在溶液中的聚集状态以及浓度淬灭作用有关。当在还原剂二硫苏糖醇作用下，胰岛素的同步荧光峰强度和位置均发生明显的变化，因此利用同步荧光光谱的变化对胰岛素分子存在形态和结构进行表征。     

检测维生素C的囊泡荧光传感器的制备

利用合成的含有识别基团苯硼酸和荧光读出基团萘的新型双亲化合物(DNMPBA)在THF/水选择性溶剂中自组织成囊泡,囊泡的相变温度为56.8℃;当向囊泡体系加维生素C时,DNMPBA囊泡中的萘生色基在345nm的荧光峰强度急剧减弱.荧光强度减弱归于所形成的硼酸酯增强了DNMPBA双亲化合物中一个氧原子孤对电子对萘生色基的淬灭作用.DNMPBA囊泡与维生素C的相互作用而导致体系荧光强度变化,使该体系有可能应用于检测生物物质如维生素C的化学传感器.     

酪氨酸的分频荧光光谱研究

酪氨酸在300nm处产生一个荧光峰,在600nm处产生一个1／2分频荧光峰,在900nm处产生一个1／3分频荧光峰,此三峰具有相似的荧光特性。根据非线性光学分频荧光原理探讨了酪氨酸分频荧光峰产生的原因。     

原小檗碱类生物碱与抗凋亡蛋白Bcl-2相互作用的光谱法研究

应用荧光光谱法研究三种原小檗碱类生物碱: 盐酸小檗碱(Berberine hydrochloride)、药根碱(Jatrorrhizine)和盐酸巴马汀(Palmatine hydrochloride)与抗凋亡蛋白Bcl-2之间的相互作用, 探讨了这三种生物碱对Bcl-2蛋白的荧光淬灭机理, 分别测定其结合常数和结合位点数. 实验结果表明, 盐酸小檗碱、药根碱和盐酸巴马汀等三种原小檗碱类生物碱都能够使Bcl-2蛋白的内源性荧光发生淬灭, 其荧光淬灭过程是静态淬灭过程. 通过比较三种生物碱与Bcl-2蛋白的相互作用, 发现随着分子柔性增强, 其与Bcl-2蛋白的结合能力增强. 因此, 增强生物碱的分子柔性有可能增强其靶向Bcl-2蛋白的能力.     

铜(Ⅱ)离子与神经红蛋白的相互作用

利用紫外可见吸收光谱、荧光光谱、同步荧光光谱及圆二色(CD)光谱研究了铜髤离子与神经红蛋白(NGB)的相互作用。结果表明,Cu2+离子使NGB在280nm处的紫外吸收增强,说明Cu2+与NGB发生了相互作用;Cu2+使NGB内源性荧光发生猝灭,其猝灭机制为静态猝灭;同步荧光光谱表明,Cu2+使色氨酸微环境的疏水性有所降低,Cu2+对NGB的作用位点更接近于色氨酸;CD光谱显示Cu2+没有引起NGB二级结构明显的变化。     

碳笼氧化物的结构和谱学性质

碳笼氧化物对碳笼的官能团化研究具有重要意义,因而激起了人们广泛的研究兴趣。本文对碳笼氧化物C₆₀O_n、C₇₀O_n、C₇₆O_n、C₇₈O_n及C₈₀O_n的结构、电子光谱、红外光谱及核磁共振谱的研究进展进行综合评述。介绍了国内外近十几年来众多研究小组的工作,并结合作者本人在此方面的理论研究成果,进一步探索碳笼氧化物的结构特点以及光谱性质的规律性。在对C₆₀及C₇₀氧化物研究结果与实验符合的基础上,预测高碳笼氧化物的结构和光谱。     

Synthesis and structural studies of complexes of Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) with substituted chalcones

Complexes of Co(II), Ni(II), Cu(II), Zn(II) and Cd(II) with 3-(2-pyridyl)-1-(2-hydroxy phenyl)-2-propen-1-one (PHPO), 3-(1-naphthyl)-1-(2-hydroxy phenyl)-2-propen-1-one (NHPO) and 3-(3,4-dimethoxy phenyl)-1-(2-hydroxy phenyl)-2-propen-1-one (DMPHPO) have been synthesized and characterized by analytical, conductivity, thermal, magnetic, infrared, electronic and electron spin resonance data. Based on analytical data the stoichiometry of the complexes has been found to be 1 : 2. The conductivity data show that all these complexes are non-electrolytes. The infrared spectral data indicate that the ligand PHPO acts as uninegative tridentately towards Co(II) and Ni(II) and bidentately with Cu(II), Zn(II) and Cd(II). Ligands like NHPO and DMPHPO act as uninegative bidentately with all the metal ions. The electronic spectral data suggest that all the Co(II) complexes and Ni(II) of PHPO complex are octahedral and all the Cu(II) and Ni(II) of NHPO and DMPHPO complex are square-planar. The complex of Zn(II) and Cd(II) are tetrahedral. ESR parameters of Cu(II) complexes have been calculated and relevant conclusions have been drawn with respect to the nature of bonds present in them.     

Chlorofluorieren mit Trifluormethylhypochlorit CF3OCl. Darstellung und spektroskopische Charakterisierung von Trifluormethyliodchloridfluorid CF3I(Cl)F

Chlorofluorinate with Trifluoromethylhypochlorite CF₃OCl. Preparation and Spectroscopic Characterization of Trifluormethyliodinechloridefluoride CF₃I(Cl)F The preparation of a new iodine(III) compound, trifluormethyliodinechloridefluoride CF₃I(Cl)F, via oxidative addition of trifluoromethylhypochlorite CF₃OCl to trifluoromethyliodide CF₃I is described. The thermolabile compound has been characterized by i.r., Raman, ¹⁹F NMR, and mass spectroscopy.     

5,10,15,20-四(对氯苯基)卟啉羟基稀土配合物的光谱研究

研究了四(对氯苯基)卟啉及其羟基稀土配合物的紫外-可见光谱、红外光谱和拉曼光谱,对主要谱带进行了经验归属,讨论了中心离子对紫外光谱和共振拉曼光谱结构敏感带频率的影响.     

Derivate von Tricyanmethan. 2. Untersuchungen über Methyltricyanmethan (1,1,1-Tricyanethan) [1]

Derivatives of Tricyanomethane. 2. Studies on Methyl Tricyanomethane (1,1,1-Tricyanoethane) The title compound H₃C? C(CN)₃ has been characterized by means of nuclear magnetic resonance, vibrational, and mass spectra.     

Diffuse reflection FTIR spectral database of dyes and pigments

24 pigments commonly used in art have been characterized by diffuse reflection infrared spectroscopy (DR). All of the compounds have also been characterized by means of infrared absorption spectroscopy to demonstrate the reliability of the DR technique. This is the first record of the use of this technique as an analytical tool in conservation science, and the results appear to be promising for the identification of unknown pigments used on historical and artwork artifacts. Although the DR technique used here is not nondestructive, it can still be usefully applied to the analysis of artwork since it requires only a very small quantity of sample for analysis.     

The role of TiO2 in the B2O3–Na2O–PbO–Al2O3 glass system

Optical and vibrational studies have been carried out on 60B₂O₃·(20−x)Na₂O·10PbO·10Al₂O₃:xTiO₂ (x=0, 1, 2, 3, 4, and 5 mol%) glasses, in order to understand the role of TiO₂ in the 60B₂O₃·20Na₂O·10PbO·10Al₂O₃ glass matrix. The X-ray patterns reveal homogeneous glasses over the entire compositional range. The absorption spectra show that the energy of the optical band gap (ΔE_opt) and Urbach's energy (E_U) decreases as TiO₂ content increases. The changes observed in the Raman and IR spectra are related to the BO₄→BO₃ back conversion effect and the appearance of “loose” BO₄⁻ groups. The data indicate that titanium ions act as a network modifier.     

Dinitramide and its salts

The vibrational and electronic spectra of dinitramide salts MN(NO₂)₂ (M=K, Na, Li, NH₄, Fe, Ag, Mn, Mg, Rb, C(NH₂)₃) have been studied. The IR and Raman spectra of solutions and melts of the salts have been satisfactorily interpreted on the basis of the anion model withC _2v symmetry. The complication of the spectra in the crystalline phase has been explained by restructurization of the anion that reduces its symmetry and makes the nitro groups nonequivalent.For part 6, seeIzv. Akad. Nauk, Ser. Khim., 1995, 113 [Russ. Chem. Bull.,44, 108 (Engl. Transl.)].Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 8, pp. 1508–1512, August, 1995.     

Preparation and investigation of the crystals formed in the buckminsterfullerene-tetraselenotetracene-carbon disulfide system

Crystals containing up to 30 wt. % CS₂, according to the data of IR and X-ray photoelectron spectroscopy, were isolated from the C₆₀-TSeT-CS₂ (TSeT is tetraselenotetracene) system. The unusually high concentration of carbon disulfide results in the complete sublimation of the crystals at a relatively high temperature (520 °C). The electron energy loss spectra of the crystals obtained were measured and analyzed.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 5, pp. 845–848, May, 1995.This work was financially supported by the Russian Foundation for Basic Research (Projects No. 93-03-18002, 93-03-18705, and 93-03-5650).