过氧化氢—次氯酸钠氧化鲁米诺发光的研究

过氧化氢－次氯酸钢可氧化碱性鲁米诺而产生强化学发光，对影响发光的诸因素及化学发光机理进行了研究，并探讨了该化学发光反应在过氧化氢测定中的应用。     

对位酚类衍生物作为化学发光分析的增强剂研究

在化学发光分析中，常常要用到碱性条件下鲁米诺与过氧化氢的反应系统，通过催化剂辣根过氧化物酶使反应顺利进行。如果再加入适当的增强剂，则灵敏度提高且发光时间延长，可改善测定的重现性。实验证明，对位酚类衍生物，如：对叔丁基苯酚，对甲苯酚的发光增强作用明显，其发光效率可上升几十倍，发光时间也获得有效延长。     

鲁米诺化学发光猝灭法测定微量狄的研究

本文利用狄对鲁米诺－过氧化氢－铬（Ⅱ）体系化学发光的熄灭效应，建立了狄的流动注射化学发光分析法。该法灵敏度高，线性范围宽，仪器设备简单，操作方便。     

碳点在鲁米诺和草酸酯化学发光体系中的研究进展

作为一种无需光、热、声、电、磁激发的自发光现象,化学发光已经在化学分析检测、冷光源、生物成像等领域得到了广泛应用.对于多数化学发光体系而言,其化学发光性质不仅取决于化学反应的底物,更与化学反应中使用的催化剂和发光中间体相关.碳点是一种新型的零维发光碳纳米材料,以其优异的物理化学性质和丰富的结构形貌已在各种化学发光体系中...     

鲁米诺-H2O2流动注射化学发光法测定乐果

在碱性介质中,乐果能够有效增强鲁米诺-H2O2体系的化学发光,据此建立了测定乐果的流动注射化学发光分析方法,并对反应的机理进行了探讨.在最佳条件下,乐果-鲁米诺-H2O2体系化学发光强度在2s内达到了最大值,乐果在5.0×10-8-1.0×10-5 g/mL范围内呈良好的线性关系,检出限(S/N=3)为1.5×10-8 g/mL.该体系应用于加标蔬菜样品测定,乐果测定回收率为108.0%-119.3%,测定偏差为2.7%-4.6%.化学发光的机理可能是由于乐果先被过氧化氢氧化生成过氧化磷酸盐,过氧化磷酸盐氧化鲁米诺生成激发态,从而产生发光.     

新的光源——化学发光

1.序言 化学发光,正象G.Brandt 1670年观察到的磷在空气中发光那样,很早就被人们发现了。目前已知发光比较强的物质有氨基苯二酰—肼~(17)(Luminol),光泽精(Lu-cigenin),洛粉碱[2、4、5-三苯基咪唑](Lophine)等,这些发光物质发蓝白色或近似绿色的蓝白色的光。使草酰氯(Oxalylchroride)和过氧化氢同各种荧光物质作用,也可得到各种颜色的发光。另外还有一些发射紫外线和红外线的发光物质。 这些发光从应用的角度来看,不论发光强度还是发光持续时间,用作一般照明,虽然困难,但仍具有很大魅力。首先因为它不需要电能,所以使用上不大受限制,象萤火     

化学发光检测光子计数的统计特性

对于增强化学发光酶联免疫分析而言，检测结果、计数效率、信噪比等重要参数不仅与光子计数装置有关，而且与光学发光体系的光子脉冲发射统计过程相关。本文实验测量了增强鲁米诺化学发光体系的光子脉冲的统计特性，并根据其脉冲发射时间统计特性对化学发光光子计数检测装置的设计进行了理论分析。研究结果表明，化学发光的光子脉冲发射间隔呈Weibull分布。     

生物发光和化学发光学术讨论会在苏州召开

1990年5月22日至24日由中国生物物理学会光生物专业委员会和中国物理学会发光分科学会联合举办的生物发光和化学发光学术讨论会在苏州举行。参加会议的代表来自全国16个省市,有一百多人,其中中青年科学工作者(包括研究生)过半数。交流的论文达120篇(宣读64篇),论文的内容覆盖面广,有生物发光、生物的超微弱发光、细胞化学发光、体表发光、化学发光和荧光标记,化学及荧光免疫分析法及测量发光仪器仪表等。从人数、论文数来看,都比1987年苏州光生物学会议增加了四倍,说明生物发光和化学发光这一新兴学科已在我国生根,并得到了迅速地发展。     

对碘酚对鲁米诺化学发光增强的试验研究

本文报道了由过氧化物酶催化鲁米诺与过氧化氢发光反应的增强发光研究。合成了增强剂对碘酚,并用对碘酚对鲁米诺与过氧化氢发光反应的光增强作用进行了试验,已经证实增强发光反应可将过氧化物酶的最低检测浓度降低到10-12～10-13mol/L水平。     

ClO^_—H2O2氧化乙醇发光的研究和应用

本文研究了ClO~--H_2O_2氧化乙醇的化学发光,对影响该发光反应的一些因素进行了试验和讨论,并依据化学发光强度与乙醇浓度间的线性关系建立了常量乙醇的化学发光测定法。     

二元酚类化合物的流动注射化学发光抑制分析法

发现邻苯二酚,对频丁基邻苯地酚,对苯二酚,３,４－二羟基苯甲对Ｃｏ＾２＋催化的鲁米诺－过氧化化学发光反应有较强的抑制作用,在此基础上建立了四种二元酚类化合物的流动 射化学发光抑制分析法。     

槲皮素化学发光反应机理探讨

本文测量了槲皮素的化学发光光谱,λmax为520nm。研究了槲皮素的化学发光反应,反应过程的荧光,动力学特性及产物的纸色谱。首次提出了槲皮素-H2O2-KOH的一种可能的化学发光反应机理。     

流动系统中鲁米诺电生化学发光(ECL)系统研究初探

本文描述了一个新的鲁米诺电生化学发光流动系统.通过微铂电极流动池,H2O2被还原为OH-以维持鲁米诺化学发光所要求的pH条件.本文对鲁米诺化学发光机理进行了初步探讨,并详细研究了ECL的最佳条件,该系统明显降低了反应噪声,优于混合反应系统,可作为液相色谱间接测定检测器.     

Luminol-Cu(Ⅱ)体系流动注射化学发光法测定维生素B2

发现了Luminol-Cu(Ⅱ)-维生素B2化学发光体系,探讨了影响化学发光反应的各个因素,建立了测定维生素B2的流动注射化学发光新方法.该方法的线性范围为2.0×10-8-2.0×10-6g/mL,检出限为8×10-9g/mL,相对标准偏差为2.3%(2.0×10-7g/mL,n=11).该方法已用于药物制剂中维生素...     

Optical Sensor for the Determination of Glucose Based on KIO4 Chemiluminescence Detection

A method to determine glucose using an optical sensor prepared by entrapping glucose oxidase into silica sol-gel column has been developed. The silica sol-gel film was coated on alumina substrate. The optical sensor is based on the chemiluminescence intensity from the reaction of periodate and hydrogen peroxide in K2CO3 medium. The effect of the ratio of water and alcohol for the preparation of TEOS sol on chemiluminescence intensity was investigated. The effects of pH of enzyme reactor, concentrations of potassium periodate and SDS, and flow rate on the chemiluminescence intensity were studied to find the optimum experimental conditions to determine glucose. The chemiluminescence intensity increased linearly with increasing glucose concentration from 5.0 x 10(-4) M to 1.0 x 10(-7) M and the detection limit was 4.0 x 10(-8) M. Interference effects from some metal ions on chemiluminescence intensity were also investigated.     

HCCI燃烧过程化学发光光谱研究

在一台经改装单缸光学发动机机上,进行不同喷油策略和进气温度条件下均质压燃(HCCI)燃烧化学发光光谱实验研究。实验保证循环供油量一定,燃用正庚烷作为燃料,转速600 r.min-1,进气压力0.1MPa,控制2个不同的进气温度:95和125℃。化学发光光谱研究结果表明,低温反应阶段化学发光很弱,主要源于甲醛光谱;低温反应后期-负温度系数区-高温反应初始阶段主要发光来源还是甲醛光谱;高温反应阶段发光主要来源于CO—O*连续谱,同时在CO—O*连续谱上出现OH,HCO,CH,HCHO谱峰;高温反应后期化学发光明显减弱。与-30°ATDC喷油相比,-300°ATDC喷油时CO—O*连续谱发光强度更大,HCO和OH生成量更多,燃烧反应进行程度更深。较高进气温度下CO—O*连续谱发光强度更大,HCO和OH生成量更多。     

Effect of cyclodextrin solutions on chemiluminescence of 10,10′-dimethyl- 9,9′biacridinium nitrate

Solutions of alpha, beta, and gamma cyclodextrin have been shown to enhance the chemiluminescence of the reaction between hydrogen peroxide and 10,10'-dimethyl-9,9'biacridinium nitrate. A 20-fold increase in chemiluminescence intensity is observed in 10^-2 M beta cyclodextrin. The enhancement is attributed to an increase in the excitation efficiency and the rate of the reaction through the inclusion of a reaction intermediate in the cyclodextrin cavity.     

Visible chemiluminescence of ammonia premixed flames and its application for flame diagnostics

We report a spatially resolved spectroscopic study of the visible chemiluminescence emission from different premixed ammonia-air-oxygen flames stabilized on a laminar flat flame burner, with equivalence ratio ranging from 0.7 to 1.35 and an O₂/N₂ ratio of 0.4. In the reaction zone of the observed flames, the visible emission was recognized to be the chemiluminescence of excited NH₂* radicals, while in the post-flame zone, two types of chemiluminescence were observed: NO₂* chemiluminescence dominated in the fuel-lean flames and NH₂* chemiluminescence dominated in the fuel-rich flames. The high-resolution spectra of the NO₂* and NH₂* chemiluminescence in the visible region (400-700 nm) were recorded. The intensity of both spectra increased gradually with wavelength. However, the NO₂*-chemiluminescence spectrum appeared to be continuous and unstructured, while the NH₂*-chemiluminescence spectrum consisted of groups of distinct emission lines. Based on the spectral feature, the ratios of the integrated chemiluminescence intensities over the 598-603 nm wavelength range to the intensities over the 586-592 nm range and 447-453 nm range were used to sense equivalence ratio. In addition, slightly different colors of the fuel-lean and fuel-rich flames were observed, due to the fact that NO₂* chemiluminescence had a relatively stronger signal in the blue region than NH₂* chemiluminescence. The difference was used to infer flame equivalence ratio using the flame images recorded by a RGB digital camera, where the ratio of the signal from the red channel to the signal from the blue channel was calculated.     

Inverted Micellar Enhanced Organic Peroxyoxalate Chemiluminescence

The use of neutral surfactant polyoxyethylene (9) dodecyl ether (AEO9) and polyoxyethylene (10) hexylphenol ether (TX-10) as an additive to enhance the chemiluminescence of peroxyoxalate reaction using bis(2-butoxycarbonyl-3,4,6-trichlorophenyl) oxalate (BBTPO) and hydrogen peroxide as the energy source in organic media with 9,10-diphenylanthracene as a fluorophore had been investigated. The oxidation of BBTPO in both AEO9 and TX-10 inverted micelles produced significantly improved intensity and efficiency of the chemiluminescence relative to that in normal organic media. It was found that in AEO9 and TX-10 inverted micellar media the enhancement of chemiluminescence was mainly due to the improvement of the reaction excitation efficiencies, but only slightly contributed by the increase of the fluorescence efficiency.     

Chemiluminescence of ozonized polyvinylchloride

Time dependences of decomposition of peroxides in samples of polyvinylchloride (PVC) ozonized under different conditions and containing different concentrations of peroxidic groups were followed by chemiluminescence method. The kinetic characteristics of the chemiluminescence reaction were determined and the mechanism accounting for the formation of hydroperoxides in PVC during ozonization was suggested.