Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice

A high yielding rice variety mutant (Oryza sativa L., Zhenhui 249) with low chlorophyll b (Chl b) has been discovered in natural fields. It has a quality character controlled by a pair of recessive genes (nuclear gene). The partial loss of Chl b in content affects the efficiency of light harvest in a light harvest complex (LHC), thus producing the difference of the exciting energy transfer and the efficiency of photochemistry conversion between the mutant and wild-type rice in photosynthetic unit. The efficiency of utilizing light energy is higher in the mutant than that in the wild-type rice relatively. For further discussion of the above-mentioned difference and learning about the mechanism of the increase in the photochemical efficiency of the mutant, the pico-second resolution fluorescence spectrum measurement with delay-frame-scanning single photon counting technique is adopted. Thylakoid membranes of the mutant and the wild-type rice are excited by an Ar^+ laser with a pulse width of 120 ps, repetition rate of 4 MHz and wavelength of 514 nm. Compared with the time and spectrum property of exciting fluorescence, conclusions of those ultrafast dynamic experiments are: 1) The speeds of the exciting energy transferred in photo-system I are faster than that in photo-system II in both samples. 2) The speeds of the exciting energy transfer of mutant sample are faster than those of the wild-type. This might be one of the major reasons why the efficiency of photosynthesis is higher in mutant than that in the wild-type rice.     

核结构模型中的两体以上相互作用（英文）

讨论了核结构模型中两体以上相互作用的可能形式。首先以基于手征微扰论并适于描述轻核的三体接触型相互作用为例,讨论了能合理描述3H 和3He 结合能的三核子接触型相互作用相关的两个低能有效耦合参数c_D 和c_E间的关系并通过4He 结合能给出了相应的物理参数区。其次通过Okubo-Lee-Suzuzki 有效相互作用方法对核多体问题在有限模型空间的求解,论证了A-体相互作用项。最后利用包含了A-体推广对力的可解模型对重核的同位素长链进行了分析。以132Sn 为核芯,通过对Sn 同位素链的计算揭示了推广对力参数G（A） 与模型价核子空间维数dim（A） 间的显著关系:G（A）=259.436 dim（A）-0.9985。这些分析结果说明,有必要对核中经手征微扰论或其它唯象理论所得到的NNN-,NNNN-,及A-体相互作用作进一步的研究。We discuss modeling of nuclear structure beyond the 2-body interaction paradigm. Our first example is related to the need of three nucleon contact interaction terms suggested by chiral perturbation theory. The relationship of the two low-energy effective coupling parameters for the relevant three nucleon contact interaction terms c_D and c_E that reproduce the binding energy of 3H and 3He has been emphasized and the physically relevant parameter region has been ilustrated using the binding energy of 4He. Further justification of A-body interaction terms is outlined based on the Okubo-Lee-Suzuki effective interaction method used in solving the nuclear many-body problem within a finite model space. The third example we use is an exactly solvable A-body extended paring interaction applied to heavy nuclei with a long isotopic chain; in particular using 132Sn as closed core system illustrates a remarkable relationship between the extended pairing strength G(A) and the size of the valence space dim(A) for the members of the Sn-isotope chain: G(A)=αdim(A)-β with α=259.436 and β =0.9985 which is actually a one parameter expression since β is practically 1. These three cases present evidence for the need of better understanding of the NNN-, NNNN-, and A-body interactions in nuclei either derived from ChPT or from a phenomenological considerations.     

Influence of Combination of Casimir Force and Residual Stress on the Behaviour of Micro-and Nano-Electromechanical Systems

Casimir force and residual stresses actually appear in over-layers or films simultaneously.The study of the behaviour of micro-and nano-electromechanical systems in the presence of Casimir force and residual stress is of significance to the design of the relevant devices.We derive analytical expression of the deflection of a bridge-shaped device under the mutual actions of Casimir force and residual stress in films.It is shown that the tensile residual stress enhances wavy behaviour of the deflection,while the compressive residual stress increases the deflection value and reduces the wavy behaviour.     

Negative bias-induced threshold voltage instability and zener/interface trapping mechanism in GaN-based MIS-HEMTs

We investigate the instability of threshold voltage in D-mode MIS-HEMT with in-situ SiN as gate dielectric under different negative gate stresses.The complex non-monotonic evolution of threshold voltage under the negative stress and during the recovery process is induced by the combination effect of two mechanisms.The effect of trapping behavior of interface state at SiN/AlGaN interface and the effect of zener traps in AlGaN barrier layer on the threshold voltage instability are opposite to each other.The threshold voltage shifts negatively under the negative stress due to the detrapping of the electrons at SiN/AlGaN interface,and shifts positively due to zener trapping in AlGaN barrier layer.As the stress is removed,the threshold voltage shifts positively for the retrapping of interface states and negatively for the thermal detrapping in AlGaN.However,it is the trapping behavior in the AlGaN rather than the interface state that results in the change of transconductance in the D-mode MIS-HEMT.     

稀土区奇A核晕带存在从转动到振动形状演化的直接证据(英文)

原子核的形状演化效应是核结构研究的重要基础问题之一。通常认为,A=160质量区的奇A核位于大形变核区域,它们的激发态能谱将呈现出典型的转动激发特征。然而,基于E-GOS曲线方法,发现随着角动量的增加,该质量区奇A核的晕带具有显著地从转动激发演化成为振动激发的形状演化现象。此外,为深入理解原子核形状演化的微观机制,采用Total-Routhian-Surface（TRS）方法针对稀土区的奇A核进行了理论计算,结果表明,165Yb和157Dy同位素在低激发态时具有稳定的长椭形变,当角动量大于0.50 MeV后,核芯的四极形变显著减小并开始产生三轴形变。The phase transition of nuclei with increasing angular momentum (or spin) and excitation energy is one of the most fundamental topics of nuclear structure research. The odd-N nuclei with A ≈160 are widely considered belonging to the well-deformed region, and their excitation spectra are energetically favored to exhibit the rotational characteristics. In this work, however, the evidence suggesting that the nuclei changes from rotation to vibration along the yrast lines as a function of spin was found. The simple method, named as E-Gamma Over Spin (E-GOS) curves, would be used to discern the evolution from rotational to vibrational structure in nuclei for various spin ranges. Meanwhile, in order to understand the band structure properties of nuclei, theoretical calculations have been performed for the yrast bands of the odd-A rare-earth nuclei within the framework of the total routhian surface (TRS) model. The TRS plots predict that the 165Yb and 157Dy isotopes have large quadrupole shapes at low spin states. At higher rotational frequency (hω~ >0.50 MeV), a clear reduction of the quadrupole deformation is indicated by the present results, and the isotopes become rigid in the γ deformation.     

轴对称奇A核系统的强耦合临界点对称性

鉴于临界点对称性方法在描述原子核形状相变中的成功应用,在强耦合方案下对原有描述偶偶核球形到长椭形状相变的临界点对称性模型X（n）（n=3,4,5）进行扩展,据此建立描述轴对称奇A核系统的SX（n）临界点对称性。通过对X（n）模型与SX（n）模型的转动谱结构进行对比分析,揭示了SX（n）临界点模型的动力学结构受模型维数n的影响较小且更接近刚性转子的模型特征,这些模型特征进一步通过检验过渡区核素150,151,152,153Sm以及172,173,174,175Os中的相关转动带结构得到了初步证实。In view of the successful application of the methods based on critical point symmetries (CPSs) in nuclear shape phase transitions, the new CPSs named SX(n) are established in this work for axially-symmetric odd-A nuclei through extending the original X(n) CPSs with n=3,4,5, which were used to describe the spherical to prolate shape phase transitions in even-even nuclei, in the strong-coupling scheme. By comparing the spectral structures in between the X(n) and the SX(n) CPSs, it is revealed that the dynamical structures of SX(n) are closer to the rigid rotor and less changed with the model dimension in comparison with X(n). Moreover, these features of SX(n) are preliminarily verified by checking the rotational structures of 150,151,152,153Sm and 172,173,174,175Os nuclei.     

Study on the negative bias temperature instability effect under dynamic stress

This paper studies negative bias temperature instability (NBTI) under alternant and alternating current (AC) stress.Under alternant stress,the degradation smaller than that of single negative stress is obtained.The smaller degradation is resulted from the recovery of positive stress.There are two reasons for the recovery.One is the passivation of H dangling bonds,and another is the detrapping of charges trapped in the oxide.Under different frequencies of AC stress,the parameters all show regular degradation,and also smaller than that of the direct current stress.The higher the frequency is,the smaller the degradation becomes.As the negative stress time is too small under higher frequency,the deeper defects are hard to be filled in.Therefore,the detrapping of oxide charges is easy to occur under positive bias and the degradation is smaller with higher frequency.     

几百兆电子伏特/核子能区原子核诱发乳胶核反应靶核蒸发碎片前后发射研究（英文）

对150 AMeV 4He-AgBr,290 AMeV 12C-AgBr,400 AMeV 12C-AgBr,400 AMeV 20Ne-AgBr及500 AMeV 56Fe-AgBr作用靶核蒸发碎片在反应前后半球内的多重数分布、多重数矩、标度方差、熵及约化熵分别进行了分析。实验结果表明,靶核蒸发碎片在前后半球内的多重数分布可以用高斯分布来描述。在前后半球内的多重数分布矩分别随秩数的增加而增加,且前后半球内多重数分布二阶矩与反应类型及束流能量无关。对于所有研究的核反应,其标度方差（一个直接描述多重数涨落的变量）值接近于1,表明在前后半球内靶核蒸发的发射存在较弱的关联。在前后半球内靶核蒸发碎片发射过程中的熵及约化熵在实验误差范围内与反应系统无关。The multiplicity distribution,multiplicity moment,scaled variance,entropy and reduced entropy of target evaporated fragments emitted in forward and backward hemispheres in 150 AMeV 4He-AgBr,290 AMeV 12C-AgBr,400 AMeV 12C-AgBr,400 AMeV 20Ne-AgBr and 500 AMeV 56Fe-AgBr interactions are investigated.It is found that the multiplicity distribution of target evaporated fragments emitted in both forward and backward hemispheres can be fitted by a Gaussian distribution.The multiplicity moments of target evaporated particles emitted in the forward and backward hemispheres increase with the order of the moment q,and the second-order multiplicity moment is energy independent over the entire energy range for all the interactions in the forward and backward hemisphere.The scaled variance,a direct measure of multiplicity fluctuations,is close to one for all the interactions,which indicate a weak correlation among the target evaporated fragments.The entropy of target evaporated fragments emitted in forward and backward hemispheres are the same respectively for all of the interactions,within experimental errors.     

Thermally stratified flow of hybrid nanofluids with radiative heat transport and slip mechanism: multiple solutions

Research on flow and heat transfer of hybrid nanofluids has gained great significance due to their efficient heat transfer capabilities.In fact,hybrid nanofluids are a novel type of fluid designed to enhance heat transfer rate and have a wide range of engineering and industrial applications.Motivated by this evolution,a theoretical analysis is performed to explore the flow and heat transport characteristics of Cu/Al₂O₃ hybrid nanofluids driven by a stretching/shrinking geometry.Further,this work focuses on the physical impacts of thermal stratification as well as thermal radiation during hybrid nanofluid flow in the presence of a velocity slip mechanism.The mathematical modelling incorporates the basic conservation laws and Boussinesq approximations.This formulation gives a system of governing partial differential equations which are later reduced into ordinary differential equations via dimensionless variables.An efficient numerical solver,known as bvp4c in MATLAB,is utilized to acquire multiple(upper and lower)numerical solutions in the case of shrinking flow.The computed results are presented in the form of flow and temperature fields.The most significant findings acquired from the current study suggest that multiple solutions exist only in the case of a shrinking surface until a critical/turning point.Moreover,solutions are unavailable beyond this turning point,indicating flow separation.It is found that the fluid temperature has been impressively enhanced by a higher nanoparticle volume fraction for both solutions.On the other hand,the outcomes disclose that the wall shear stress is reduced with higher magnetic field in the case of the second solution.The simulation outcomes are in excellent agreement with earlier research,with a relative error of less than 1%.     

SD配对壳模型对质量数A~130区原子核的形状渡越研究(英文)

利用SD配对壳模型研究了质量数A~130区原子核的低激发态性质。为了减少参数,模型采用了等强度对力,取质子和中子对力强度等同近似,而非同类核子间的四极-四极相互作用强度取为同类核子间四极-四极相互作用强度的两倍。计算结果显示,模型的振动-转动和振动-γ不稳定运动的形状渡越特征与相互作用玻色子模型对应的形状相变特征基本吻合,从而说明SD配对壳模型是壳模型的一种合理近似。The SD-pair shell model is applied to analyze the evolution of low-lying states of even-even nuclei in A~130 mass region. In the model, the pairing and the quadrupole-quadrupole interactions are taken into account. The results show that there are clear signatures of the crossover from vibrational to rotational or from vibrational to the γ-soft shape phase.     

利用原子吸收光谱法分析盐胁迫对野生披碱草矿质元素吸收和积累的影响

应用原子吸收光谱法测定了两份耐盐性不同的野生披碱草种质材料在不同盐浓度胁迫条件下根组织和叶组织中的大量元素(Na,K,Ca,Mg)和微量元素(Cu,Fe,Mn,Zn)含量,并对野生披碱草种质材料矿质元素吸收和积累的选择性与耐盐性的关系进行了分析,结果表明：随着盐胁迫浓度的升高,野生披碱草种质材料叶和根组织中Na含量不断增加,耐盐材料HS叶组织中的Na含量显著低于敏盐材料LS(P<0.05);而K和Ca含量呈下降的趋势,在高浓度盐胁迫下,耐盐材料HS中两种元素的含量显著高于敏盐材料LS(P<0.05);微量元素Fe和Zn在野生披碱草材料根、叶组织中的含量升高,Cu在根组织中的含量没有变化,但是在叶组织中升高,而Mn在根、叶组织中的含量都呈下降的趋势,耐盐材料HS叶组织和根组织中Zn和Fe含量低于敏盐材料LS,Mn含量显著高于敏盐材料LS(P<0.05)。     

利用光诱导延迟荧光的植物盐胁迫生理监测

利用自制的便携式延迟荧光(Delayed Fluorescence,DF)探测系统,以早熟18号大豆品种为实验材料,研究了盐胁迫对植物叶绿体DF特性的影响.结果发现在单纯的盐胁迫和盐胁迫伤害程度被调控缓解时,绿色植物叶片光诱导DF强度的变化都能很好地反应植物叶片光合性能的变化.因此,可以用DF强度的变化来表征盐胁迫对植物的伤害程度.     

Depletion of arginine in yeast cells decreases the resistance to hydrostatic pressure

High hydrostatic pressure (HP) inhibits growth and inactivates microorganisms by destabilizing non-covalent molecular interactions. Arginine contributes to stress resistance because it has a guanidine side chain, which assists in the refolding of aggregated proteins. We attempted to analyze the contribution of arginine to high HP stress using a pressure-sensitive mutant strain of Saccharomyces cerevisiae and a metabolomics approach. Our results showed that the content of 136 out of 250 detected metabolites differed in the mutant and parent strains. Decreased metabolites were involved in the tricarboxylic acid cycle and arginine biosynthesis. The expression of genes contributing to arginine biosynthesis was significantly lower in the mutant strain than in the parent strain. When arginine was supplemented to the medium, the mutant strain showed more tolerance to pressure. These results suggest that yeast cells survived due to the contribution of arginine to high pressure resistance. This indicates that depletion of arginine caused by decreased activity of the biosynthesis pathway confers sensitivity to HP.     

23Na and (1)H NMR microimaging of intact plants

(23)Na NMR microimaging is described to map, for the first time, the sodium distribution in living plants. As an example, the response of 6-day-old seedlings of Ricinus communis to exposure to sodium chloride concentrations from 5 to 300 mM was observed in vivo using (23)Na as well as (1)H NMR microimaging. Experiments were performed at 11.75 T with a double resonant (23)Na-(1)H probehead. The probehead was homebuilt and equipped with a climate chamber. T(1) and T(2) of (23)Na were measured in the cross section of the hypocotyl. Within 85 min (23)Na images with an in-plane resolution of 156 x 156 micrometer were acquired. With this spatial information, the different types of tissue in the hypocotyl can be discerned. The measurement time appears to be short compared to the time scale of sodium uptake and accumulation in the plant so that the kinetics of salt stress can be followed. In conclusion, (23)Na NMR microimaging promises great potential for physiological studies of the consequences of salt stress on the macroscopic level and thus may become a unique tool for characterizing plants with respect to salt tolerance and salt sensitivity.     

声波刺激对铁皮石斛过氧化物酶同工酶基因表达的影响

从基因表达水平探索了植物适应物理应力刺激时可能作出的反应机制。以铁皮石斛组培苗为实验材料,以特定的声波(声强100dB、频率1000Hz)刺激为应力源,检测比较了声波刺激对铁皮石斛过氧化物酶(POD)同工酶酶谱及各酶带百分含量的影响;通过设计引物扩增出铁皮石斛POD基因片段,以此为探针,利用Northern点杂交技术检测分析了声波刺激对铁皮石斛POD基因表达的影响。结果显示,声波刺激没有引起铁皮石斛产生新的酶带,但促进了其POD同工酶酶量的提高;声波刺激对铁皮石斛POD基因表达的影响与其同工酶酶谱及其总RNA量的变化基本一致。表明声波刺激对植物POD同工酶基因表达有激活作用,可能引起植物某些基因转录和表达水平的变化。     

植物个体远程辐射信号早期传递过程的研究体系创建

自2005年以来,活体中的远程辐射旁效应逐渐成为辐射生物学的研究热点,然而其早期信号传递过程的相关研究却鲜见报道,主要原因是由于早期信号传递过程研究所需的旁区与辐射区的自由分离与组合在动物模型上无法实现。本研究是基于植物个体可以切割和嫁接的特性,借鉴离体细胞培养基转移方法研究旁效应早期过程的思想,在植物个体上实现旁区与辐照区的"分离"与"组合",构建了一种研究个体远程辐射信号早期传递过程的植物实验体系。具体是以模式植物拟南芥菜转基因系(AtRAD54promoter∶∶GUS)为材料,同源重组修复相关基因AtRAD54表达水平为生物学检测终点,人为地将辐照区的组织(或器官)与旁区部分"分离"或"嫁接",通过测定旁区组织(或器官)的AtRAD54基因表达水平变化,研究其辐射信号传递的早期过程。该研究体系的创建为活体旁效应早期过程的研究提供了一种可行的研究方法。     

电场对PtO2/MoS2范德瓦尔斯异质结电子结构调控

本文利用基于密度泛函理论的第一性原理计算方法研究了外电场对PtO2/MoS2范德瓦尔斯异质结电子结构的调控,发现当层间距d=2.83Å时异质结结构最稳定,且表现为Ⅱ型间接带隙半导体,其带隙为0.68 eV。通过施加垂直平面方向电场可有效调控PtO2/MoS2异质结电子结构,当外电场为-1 V/Å时,发生半导体-金属相变。这些研究结果表明PtO2/MoS2异质结在新型二维材料光电纳米器件方面具有广泛应用前景。     

Transition probabilities for sulfur lines from wall-stabilized arc measurements

A new type of analysis has been developed to determine transition probabilities of spectral lines emitted from optically thin, multi-element, arc plasmas in local thermodynamic equilibrium (LTE). Provided the ionization energy of the element of interest is considerably lower than that of the other elements, this method is independent of demixing effects for a fairly wide temperature range. The technique has been applied to spectroscopic data obtained from wall-stabilized arcs operated at atmospheric pressure, first in a mixture of argon and sulfur hexafluoride (SF₆), and second in SF₆. A trace of hydrogen is added in both experiments in order to determine the electron density from the measuredHβ hydrogen line width. The experimental data do not contradict the assumption of LTE. The transition probabilities for the SI 4695 Å, SI 5279 Å, SII 5320 Å, and SII 5454 Å lines are in good agreement with those listed in the NBS critical data compilation.