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A chemiluminescence (CL) flow system is described for the determination of isoniazid based on its enhancement on the chemiluminescence (CL) emission produced upon mixing a hexacyanoferrate(III) solution with an alkaline luminol solution. The system responds linearly to isoniazid concentration in the range 0–1 mg/L with a detection limit (3σ) of 0.03 μg/L, relative standard deviation (RSD) of 1.2% for 0.1 mg/L isoniazid (n = 11). The system has been successfully applied to the determination of isoniazid in pharmaceutical preparations. 相似文献
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A chemiluminescence (CL) flow system is described for the determination of isoniazid based on its enhancement on the chemiluminescence (CL) emission produced upon mixing a hexacyanoferrate(III) solution with an alkaline luminol solution. The system responds linearly to isoniazid concentration in the range 0-1 mg/L with a detection limit (3sigma) of 0.03 microg/L, relative standard deviation (RSD) of 1.2% for 0.1 mg/L isoniazid (n = 11). The system has been successfully applied to the determination of isoniazid in pharmaceutical preparations. 相似文献
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A chemiluminescence system is described for the determination of nitrite ion based on new designs for an ozone generator, liquid-gas separator and chemiluminescence reaction cell. The method is based on the gas-phase chemiluminescence reaction between ozone and nitric oxide, which is generated from the reduction of nitrite with iodide in sulfuric acid solution. The efficiency of the system was evaluated by investigation of the analytical performance characteristics of the system for nitrite determination in batch and flow injection procedures. Under optimal conditions, the chemiluminescence response of the system was linear against the nitrite concentration over the range 1 to 1 × 104 ng ml?1 in the batch procedure and 10 to 5 × 103 ng ml?1 in the flow injection procedure, with detection limits of 1 and 10 ng ml?1, respectively. The method is highly selective and allows for the determination of nitrite in the presence of high concentrations of several cationic, anionic and nitrogen containing species. It has been successfully applied to the analysis of nitrite in natural water and soil extracts. 相似文献
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在NaOH-NaHCO3介质中,铁氰化钾氧化西咪替丁产生快速化学发光反应,0.5 s后发光达到最大,2 s后迅速衰减至零。本文结合流动注射技术,建立了一种化学发光测定西咪替丁的新方法。针对这一快速发光反应,设计了与之相应的管路系统和最短的反应管道来捕捉最大化学发光信号,发光强度与西咪替丁质量浓度在5×10-7~1×10-4g/mL范围内呈线性关系,检出限为1.1×10-7g/mL。对5×10-6g/mL西咪替丁进行11次平行测定,相对标准偏差为1.8%。本法已用于西咪替丁片剂的测定。 相似文献
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N-bromosuccinimide-fluorescein based sensitive flow-injection chemiluminescence determination of phenformin. 总被引:1,自引:0,他引:1
A novel and highly sensitive method for the determination of phenformin over the range of 6 x 10(-9) - 1 x 10(-5) g ml(-1) in pharmaceutical formulations with flow-injection chemiluminescence (CL) detection is proposed. The method is based on the CL produced during the oxidation of N-bromosuccinimide (NBS) in an alkaline medium in the presence of fluorescein as an effective energy transfer agent. The use of cetyltrimethylammonium bromide (CTAB) as a sensitizer enhances the signal magnitude by about 100 times. The detection limit is 2 x 10(-9) g ml(-1) (3sigma) with a relative standard deviation of 2.3% (n = 11) at 1 x 10(-7) g ml(-1) phenformin. Ninety samples can be determined per hour. The method was evaluated by carrying out a recovery study and by the analysis of commercial formulations. The obtained results compared well with those by an official method, and demonstrated good accuracy and precision. The possible CL mechanism of the proposed system was also briefly analyzed. 相似文献
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A novel chemiluminescence (CL) method was established for two anticancer drugs, adriamycin (ADM) and mitomycin (MMC), based on potassium permanganate oxidation in the presence of formaldehyde. The sensitized CL emission mechanism was developed by comparing the fluorescence emission with CL spectra. Illuminant was the singlet state bi-molecule oxygen, 1O2 1O2 (1Δg 1Δg), from 1O2 (1Δg) which was produced in the reaction system, and emitted CL spectra at 639 nm or 649 nm. The presence of formaldehyde may accelerate the generation of 1O2 (1Δg) and sensitized CL emission. The optimum conditions for CL emission were investigated and optimized. The relationships between the relative CL intensity and the concentration of the studied analytes found to be linear. The detection limit was 3 × 10?8 g ml?1 for ADM and 3 × 10?9 g ml?1 for MMC. The relative standard deviations are 2.2% and 1.8% for determinations of ADM at 2.0 × 10?6 g ml?1 and MMC at 2.0 × 10?7 g ml?1, respectively. The proposed sensitized CL system was successfully applied to the determination of ADM and MMC in their injections with satisfactory results. 相似文献
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Ultrasound-enhanced flow injection chemiluminescence for determination of hydrogen peroxide 总被引:1,自引:0,他引:1
Greenway GM Leelasattarathkul T Liawruangrath S Wheatley RA Youngvises N 《The Analyst》2006,131(4):501-508
A novel ultrasonic flow injection chemiluminescence (FI-CL) manifold for determining hydrogen peroxide (H2O2) has been designed and evaluated. Chemiluminescence obtained from the luminol-H2O2-cobalt(II) reaction was enhanced by applying 120 W of ultrasound for a period of 4 s to the reaction coil in the FI-CL system and this enhancement was verified by comparison with an identical manifold without ultrasound. The system was developed for determining ultra-trace levels of H2O2 and a calibration curve was obtained with a linear portion over the range of 10-200 nmol L(-1) H2O2 (correlation coefficient 0.9945). The detection limit (3sigma) and the quantification limit (LOQ) were found to be 1 x 10(-9) and 3.3 x 10(-9) mol L(-1) respectively and the relative standard deviation was 1.37% for 2 x 10(-7) mol L(-1) H2O2 (n = 10). The method was applied to the determination of trace amounts of H2O2 in purified water and natural water samples without any special pre-treatments. 相似文献
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The emission produced by sulfite on oxidation by permanganate in acidic solution in the presence of riboflavin phosphate or brilliant sulfaflavine is used to determine 0.9–35 ng of sulfite. Only sulfide and thiosulfate also give emissions. 相似文献
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流动注射邻菲啰啉化学发光体系测定羟自由基 总被引:3,自引:0,他引:3
利用Vitc-CuSO4-H2O2产生的羟自由基,建立流动注射邻菲口罗啉化学发光体系测定羟自由基的产生及物质对羟自由基的清除能力。体系中各种物质浓度的最佳组合是:邻菲口罗啉1.0×10-3mol/L(pH 8.2硼砂-硼酸缓冲液配制、内含CTMAB浓度为5.0×10-3mol/L)、Cu2+1.5×10-3mol/L、H2O2体积分数0.6%、抗坏血酸1.5×10-3mol/L。硫脲清除羟自由基的量效关系Y=25.0009ln(x)+65.3120,r=0.9988,IC50=0.542 mmol/L。龙井茶水提液对羟自由基具有较强的清除作用。 相似文献
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Carmen Gómez-Benito Sagrario Torres-Cartas 《International journal of environmental analytical chemistry》2013,93(2):152-165
In this work, a sensitive flow injection chemiluminescence (FI-CL) method for the determination of nematicide Fenamiphos in a rapid and simple way is proposed. Fenamiphos is first photodegraded in basic medium. These photofragments react with Ce(IV) providing the chemiluminescence signal. To the authors’ knowledge, no chemiluminescence method has been described in the literature for the determination of the nematicide Fenamiphos. All physical and chemical parameters in the flow injection chemiluminescence system were optimized in order to obtain the best sensitivity, selectivity and sample throughput. Before the injection of the sample in the FI-CL system, a preconcentration step with solid phase extraction C18 cartridges was performed. By applying solid phase extraction (SPE) to 250?mL of standard (final volume 10?mL), the linear dynamic range was between 3.4 and 60?µg?L?1, and the detection limit was 1?µg?L?1. When SPE was applied to 500?mL of standard (final volume 10?mL), the detection limit was 0.5?µg?L?1. These detection limits are below the emission limit value established by the Spanish Regulations of the Hydraulic Public Domain for pesticides (50?µg?L?1) and of the same order as the limit established for total pesticides (0.5?µg?L?1) at European Directive on the quality of water for human consumption. The sample throughput was 126 hour?1. Intraday and interday coefficients of variation were below 10% in all cases. No interference was registered in presence of usual concentrations of anions, cations and other organophosphorus pesticides. The method was successfully applied to the analysis of environmental water samples, obtaining recoveries between 96 and 107.5%. 相似文献
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在酸性介质中,木犀草素在KMnO4-HCHO体系中产生化学发光,据此建立了一种简便、快速测定木犀草素的化学发光新方法。化学发光强度与木犀草素的浓度在5.0×10-8~1.5×10-5g/mL范围内呈现出良好的线性关系。其检测限(3σ)为3.0×10-8g/mL,对8.0×10-7g/mL的木犀草素溶液进行11次平行测定,相对标准偏差为2.8%。方法可应用于实际样品和合成样品中木犀草素的测定。并探讨了反应的机理。 相似文献
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A sensitive flow-injection (FI) chemiluminescence (CL) for the determination of urapidil is described in this paper. It is
based on the enhancement effect of urapidil on the CL reaction between luminol and hydrogen peroxide. The increment of CL
intensity is proportional to the concentration of urapidil in the range 0.1−10 ng/mL (R
2=0.9986), with a detection limit (3σ) of 0.03 ng/mL. The whole process, at a flow rate of 2.0 mL/min, including sampling and
washing, could be completed in 0.5 min, and the relative standard deviation (RSD) at the concentration of 0.1, 1.0, and 10.0
ng/mL was less than 3.0% (n = 5). The proposed method has been successfully applied for the determination of urapidil in pharmaceutical preparation,
human urine, and serum.
The text was submitted by the authors in English. 相似文献
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A flow-injection manifold for the determination of plasma glucose (0–25 mM), incorporating a dialysis unit and a wall-coated enzyme reactor, is described. Spectrophotometric and chemiluminescence methods of detection are compared in terms of precision, accuracy, sample throughput, cost and reliability, and the results obtained from both methods are compared with those obtained from an electrochemical star analyser. 相似文献