曙红Y-Triton X-100疏水性氢键纳米微粒的吸收、荧光及共振瑞利散射光谱及其分析应用

在pH2.4～2.8的酸性介质中,曙红Y分子(H2L)取代水分子而与Triton X-100形成氢键缔合物.该疏水性的氢键缔合物,在水相的"挤压"作用和范德华力的作用下,能进一步聚集形成纳米微粒.此时将引起吸收光谱的变化和荧光猝灭,并导致共振瑞利散射(RRS)显著增强,为建立褪色分光光度法、荧光猝灭法和共振瑞利散射法测定Triton X-100创造了条件.三种方法均有较高的灵敏度.其中以RRS法灵敏度最高,对于Triton X-100的检出限为20.6ng/mL.本文研究了曙红Y与Triton X-100相互作用的适宜条件和对吸收、荧光和RRS光谱的影响.考察了共存物质的影响,表明方法有良好的选择性.发展和建立了灵敏、简便、快速测定Triton X-100的分光光度、荧光猝灭法和RRS新方法.文中还结合红外光谱、透射电子显微镜技术和量子化学方法对曙红Y-Triton X-100氢键缔合物及纳米微粒的形成以及对相应的光谱特性的影响进行了讨论,并研究了方法在环境分析中的应用.     

The interaction between furosemide-palladium (II) chelate and basic triphenylmethane dyes by resonance Rayleigh scattering spectra and resonance non-linear scattering spectra and their analytical applications

In pH 4.5–7.0 Britton-Robinson buffer solution, furosemide (FUR) reacted with Pd (II) to form a 1:1 anionic chelate. This chelate could further react with such basic triphenylmethane dyes (BTPMD) as ethyl violet (EV), crystal violet (CV), methyl violet (MV), methyl green (MeG) and brilliant green (BG) to form 1:1 ion-association complexes. This not only resulted in the change of absorption spectra, but also led to the significant enhancement of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS). The maximum RRS wavelengths were located at 324 nm for the EV, CV and MV system, and 340 nm for the BG and MeG system. The maximum SOS wavelengths were located at 550 nm for the EV, CV, BG and MeG system, and 530 nm for the MV system. The maximum scattering peaks of all the systems were at 392 nm for FDS. The enhanced RRS, SOS and FDS intensities were directly proportional to the concentration of FUR. The detection limits for the different dye systems were 0.3–4.9 ng mL^?1 for the RRS method, 3.2–33.1 ng mL^?1 for the SOS method and 9.0–85.7 ng mL^?1 for the FDS method. These methods could be used for the determination of trace amounts of FUR. The effects of the formation of ternary ion-association complexes on the spectral characteristics and intensities of absorption, RRS, SOS and FDS have been investigated. The optimum conditions of these reactions, the influencing factors and the analytical properties have been tested. The influences of coexisting substances were tested by RRS method and the results showed that this method exhibited a high sensitivity. Based on the aforementioned research, the highly sensitive, simple and rapid methods for the determination of trace amounts of FUR by resonance light scattering technique have been established, which could be applied to the determination of FUR in tablet, injection, human serum and urine samples. The composition and structure of the ternary ion-association complex and the reaction mechanism were discussed.     

氯霉素、甲砜霉素与曙红Y的共振瑞利散射光谱及应用

在pH 1.30的酸性介质中,曙红Y(EOSY)分别与氯霉素(CHP)、甲砜霉素(TAP)相互作用形成离子缔合物,使共振瑞利散射(RRS)显著增强并产生新的RRS光谱。CHP–EOSY体系的最大RRS峰位于313nm,线性范围为0.015～0.32 mg.L-1,检出限为0.013 mg.L-1;TAP–EOSY体系的最大RRS峰位于314nm,线性范围为0.018～0.39 mg.L-1,检出限为0.012 mg.L-1。据此发展了以曙红Y为探针,用共振瑞利散射法测定氯霉素、甲砜霉素的方法。方法简便快速,有较高灵敏度,可用于实际样品中氯霉素、甲砜霉素的测定。     

阳离子表面活性剂与曙红Y的荧光反应及其分析应用

研究阳离子表面活性剂(CSAA)在水溶液中与曙红Y的荧光反应, 发现当CSAA单体与曙红Y形成离子缔合物时, 荧光发生猝灭, 而CSAA胶束与曙红Y作用又会出现一个新的、更强的荧光。荧光猝灭反应具有很高的灵敏度, 对于不同的CSAA, 其检测限在6.6-12.0ng/mL之间, 可用于痕量CSAA的测定。此外, 荧光猝灭和新荧光的产生也为研究表面活性剂和荧光染料在溶液中的存在状态提供了新的途径。还研究了反应体系的荧光特征、适宜条件并讨论了反应机理。     

Study on the interaction between torasemide and 12-tungstophosphoric acid by resonance Rayleigh scattering and resonance nonlinear scattering spectra and its analytical applications

In pH 0.6-1.1 HCl-NaAc buffer solution, torasemide (TOR) reacted with TP to form a 3:1 ion-association complexes. As a result, not only the absorption spectra were changed, but also the intensities of resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) were enhanced greatly. The maximum RRS, SOS and FDS wavelengths were located at 370, 333, 776 nm, respectively. Under given conditions, the intensities of RRS, SOS and FDS were all directly proportional to the concentration of TOR. The detection limits of RRS, SOS and FDS were 0.7173 ng mL(-1), 7.007 ng mL(-1) and 10.90 ng mL(-1). The optimum conditions and the effects of coexisting substances on the reaction were investigated. The results showed that the method had good selectivity. Therefore, a highly sensitive, simple and quick method has been developed for the determination of TOR. The method can be applied satisfactorily to the determination of TOR in tablets and urine samples.     

Study on the absorption and fluorescence and resonance Rayleigh scattering spectra of Cu (II)-fluoroquinolone chelates with erythrosine and their applications

In pH 4.2-5.0 Britton-Robinson buffer solution medium, fluoroquinolone antibiotics (FLQs), such as ciprofloxacin (CIP), norfloxacin (NOR), ofloxacin (OF), levofloxacin (LEV), lomefloxacin (LOM), and sparfloxacin (SPA), react with Cu (II) to form chelate cations, which further bind with erythrosine to form the ion association complexes. They can result in the changes of the absorption spectra. Simultane- ously, erythrosine fades obviously and the maximum fading wavelength is located at 526 nm. The fad- ing reactions have high sensitivities. Thus, new spectrophotometries of determination for these drugs are developed. The ion-association reactions result in the quenching of fluorescence, which also have high sensitivities. The detection limits for six antibiotics are in the range of 7.1-12.2 μg·L?1. Furthermore, the reactions can result in the enhancement of resonance Rayleigh scattering (RRS). The maximum scattering peaks of six ion-association complexes are located at 566 nm, and there are two small RRS peaks at 333 nm and 287 nm. The detection limits for fluoroquinolone antibiotics are in the range of 1.70 -3.10 μg·L?1 for RRS method. Among the above three methods, the RRS method has the highest sen- sitivity. In this work, we investigated the spectral characteristics of the absorption, fluorescence and RRS, the optimum conditions of the reactions, and the properties of the analytical chemistry. In addi- tion, the mechanism of reactions were discussed by density function theory (DFT) and AM1 methods.     

Study on the resonance Rayleigh scattering spectra and resonance non-linear spectra of congo red-amikacin system and its analytical application

The interaction between congo red (CR) and amikacin (AMK) was studied by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS) combining with absorption spectrum. In a weak acidic medium, CR combined with AMK to form an ion association complex with the composition ratio of 1∶1 by electrostatic interaction, hydrophobicity and charge transferring effect. As a result, the new spectra of RRS, FDS, and SOS appeared and their intensities were enhanced greatly. The maximum wavelengths of RRS, FDS and SOS were located at 563 nm, 475 nm and 940 nm, and the scattering intensities were proportional to the concentration of AMK. These three methods have very high sensitivities, and the detection limits were 4.0 ng·mL?1 for RRS, 3.6 ng·mL?1 for FDS and 1.9 ng·mL?1 for SOS, respectively. At the same time, the methods have better selectivity. A new method for the determination of trace amounts of AMK with congo red by resonance scattering technique has been developed. The recovery for the determination of AMK in blood serum and urine sample was between 95.5% and 105.5%. In this study, the properties, such as enthalpy of formation, charge distribution and mean polarizability, were calculated by AM1 quantum chemistry method. In addition, the reaction mechanism and the reasons for the enhancement of scattering spectra were discussed.     

Study on the resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectra of the interactions of palladium(II)-ceftriaxone chelate with anionic surfactants and their analytical applications

In pH 1.8-2.9 Britton-Robinson (BR) buffer medium, ceftriaxone (CTRX) can react with palladium(II) (Pd(II)) to form 1:2 cationic chelate, which can further react with anionic surfactants (AS) such as sodium lauryl sulfonate (SLS), sodium dodecyl sulfate (SDS) and sodium dodecylbenzene sulfonate (SDBS) to form 1:3 ion-association complexes. As a result, the resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) were enhanced greatly. The maximum RRS, SOS and FDS wavelengths of three ion-association complexes were located at 335 nm, 560 nm and 390 nm, respectively. The increments of scattering intensity (DeltaI) were directly proportional to the concentrations of CTRX in certain ranges. The detection limits (3sigma) of CTRX for SLS, SDBS and SDS systems were 1.8 ng ml(-1), 2.3 ng ml(-1) and 2.3 ng ml(-1) (RRS method), 4.9 ng ml(-1), 7.4 ng ml(-1) and 4.7 ng ml(-1) (SOS method) and 6.8 ng ml(-1), 7.3 ng ml(-1) and 9.1 ng ml(-1) (FDS method), separately. The sensitivity of RRS method was higher than those of SOS and FDS methods. The optimum conditions of RRS method and the influence factors were investigated, and the composition of ion-association complexes and the reaction mechanism were discussed also. The effects of foreign substances were tested and it showed that the method has a good selectivity. Based on the ion-association reaction, the sensitive, simple and rapid methods for the determination of CTRX have been developed.     

Study on the interaction of trypsin with some DNAs and their analytical application by resonance Rayleigh scattering spectra

When trypsin reacts with Herring sperm DNA (hsDNA), Salmon sperm DNA (sDNA), and Calf thymus DNA (ctDNA) to form a complex, the resonance Rayleigh scattering (RRS) was remarkably enhanced and new RRS spectra appear. These new spectra have similar characteristics of RRS spectra. The maximum RRS peaks are at 307 nm (hsDNA, sDNA) and 290 nm (ctDNA), and other peaks are at 350 nm. The scattering intensity is proportional to the concentration of DNA or trypsin; so this intereaction can be used to determine trypsin using DNA or DNA using trypsin. In the determination of DNA using trypsin, the linear ranges for hsDNA, sDNA, and ctDNA are 0–2.3, 0–2.5, and 0–1.9 μg·mL⁻¹, and the detection limits are 0.4, 0.7, and 1.1 ng·mL⁻¹, respectively. In the determination of trypsin using hsDNA, the linear range is 0–30.0 μg·mL⁻¹, and the detection limit is 39.0 ng·mL⁻¹. In this paper, the intereaction conditions were optimized. The affecting factors, chemical properties of the complex, and the composition ratio of trypsin with DNA were investigated. Using trypsin as RRS probe, a sensitive method for the determination of trace amounts of DNA was developed. Translated from Chemical Journal of Chinese Universities, 2006, 27(3) (in Chinese)     

Effects of interaction of folic acid with uranium (VI) and basic triphenylmethane dyes on resonance Rayleigh scattering spectra and their analytical applications

In pH 4.2-4.8 HAc-NaAc buffer solution, folic acid (FA) could react with uranium (VI) to form a 2:1 anionic chelate which further reacted with some basic triphenylmethane dyes (BTPMD) such as Ethyl Violet (EV), Methyl Violet (MV) and Crystal Violet (CV) to form 1:2 ion-association complexes. As a result, not only the absorption spectra were changed, but also the intensities of resonance Rayleigh scattering (RRS) were enhanced greatly and the new RRS spectra were observed. The maximum RRS wavelengths were located at 328 nm for EV system, 325 nm for MV system and 328 nm for CV system. The fading degree (ΔA) and RRS intensities (ΔI) of three systems were different. Under given conditions, the ΔA and ΔI were all directly proportional to the concentration of FA. The linear ranges and the detection limits of RRS methods were 0.0039-5.0 μg mL⁻¹ and 1.2 ng mL⁻¹ for EV system, 0.0073-4.0 μg mL⁻¹ and 2.2 ng mL⁻¹ for MV system, 0.014-3.5 μg mL⁻¹ and 4.7 ng mL⁻¹ for CV system. The RRS methods exhibited higher sensitivity, so they are more suitable for the determination of trace FA. The optimum conditions, the influencing factors and the effects of coexisting substances on the reaction were investigated. The method can be applied to the determination of FA in serum and urine samples with satisfactory results. The structure of the ternary ion-association complex and the reaction mechanism were discussed in this work.     

Study on the interaction between fluoroquinolones and erythrosine by absorption, fluorescence and resonance Rayleigh scattering spectra and their application

In pH 4.4-4.5 Britton-Robinson (BR) buffer solution, fluoroquinolone antibiotics (FLQs) including ciprofloxacin (CIP), norfloxacin (NOR), levofloxacin (LEV) and lomefloxacin (LOM) could react with erythrosine (Ery) to form 1:1 ion-association complexes, which not only resulted in the changes of the absorption spectra and the quenching of fluorescence, but also resulted in the great enhancement of resonance Rayleigh scattering (RRS). These offered some indications of the determination of fluoroquinolone antibiotics by spectrophotometric, fluorescence and resonance Rayleigh scattering methods. The detection limits for fluoroquinolone antibiotics were in the range of 0.097-0.265 microg/mL for absorption methods, 0.022-0.100 microg/mL for fluorophotometry and 0.014-0.027 microg/mL for RRS method, respectively. Among them, the RRS method had the highest sensitivity. In this work, the spectral characteristics of the absorption, fluorescence and RRS, the optimum conditions of the reactions and the properties of the analytical chemistry were investigated. The methods have been successfully applied to determination of some fluoroquinolone antibiotics in human urine samples and tablets. Taking CIP-Ery system as an example, the charge distribution, the enthalpy of formation and the mean polarizability were calculated by density function theory (DFT) method. In addition, the reasons for the enhancement of scattering spectra were discussed.     

Resonance Rayleigh scattering, frequency doubling scattering and second-order scattering spectra of the heparin-crystal violet system and their analytical application

In pH 6.0-11.2 Britton-Robinson buffer solution, binding of heparin with crystal violet (CV) can result in a significant enhancement of resonance Rayleigh scattering (RRS) and resonance non-linear scattering, such as frequency doubling scattering (FDS) and second-order scattering (SOS). Their maximum scattering wavelengths, λ_ex/λ_em, appear at 492 nm/492 nm for RRS, 984 nm/492 nm for FDS and 492 nm/984 nm for SOS, respectively. The optimum conditions of the reaction, the influencing factors and the relationship between the three scattering intensities and the concentration of heparin have been investigated. New methods for the determination of trace amounts of heparin based on the RRS, FDS and SOS methods have been developed. The methods exhibit high sensitivities, the detection limit for heparin is 2.9 ng ml⁻¹ for the RRS method, 3.5 ng ml⁻¹ for the FDS method and 3.3 ng ml⁻¹ for the SOS method. The methods have good selectivity and were applied to the determination of heparin in heparin sodium injection samples with satisfactory results.     

Resonance Rayleigh scattering spectra, non-linear scattering spectra of tetracaine hydrochloride-erythrosin system and its analytical application

The interaction between erythrosine (ET) and tetracaine hydrochloride (TA) was studied by resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS) and second-order scattering (SOS) combining with absorption spectrum. In a weak acidic medium of Britton-Robinson (BR) buffer solution of pH 4.5, erythrosine reacted with tetracaine hydrochloride to form 1:1 ion-association complex. As a result, the new spectra of RRS, SOS and FDS appeared and their intensities enhanced greatly. The maximum peaks of RRS, SOS and FDS were at 342 nm, 680 nm and 380 nm, respectively. The intensities of the three scattering were directly proportional to the concentration of TA in the range of 0.008-4.2 microg mL(-1) for RRS, 0.027-4.2 microg mL(-1) for SOS and 0.041-4.2 microg mL(-1) for FDS. The methods had very high sensitivities and good selectivities, and the detection limits were 0.003 microg mL(-1) for RRS, 0.008 microg mL(-1) for SOS and 0.012 microg mL(-1) for FDS, respectively. Therefore, a new method was developed to determinate trace amounts of TA. The recovery for the determination of TA in blood serum and urine samples was between 97.0% and 103.8%. In this study, mean polarizability was calculated by AM1 quantum chemistry method. In addition, the reasons for the enhancement of scattering spectra and the energy transfer between absorption, fluorescence and RRS were discussed.     

Study on the resonance Rayleigh scattering spectra of the interactions of palladium (II)-cephalosporins chelates with 4,5-dibromofluorescein and their analytical applications

In pH 2.8-3.8 BR buffer medium, the third generation cephalosporin antibiotics (TGCs) such as ceftazidime (CZD), ceftriaxone (CTRX), cefoperazone (CPZ), and cefotaxime (CFTM) react with palladium(II) (Pd(II)) to form 1:2 yellowish-brown cationic chelates, which further react with 4, 5-dibromofluorescein (DBF) to form 1:3 brown ion-association complexes. As a result, not only the spectra of absorption and fluorescence are changed, but also the resonance Rayleigh scattering (RRS) is enhanced greatly and the new RRS spectra are observed. The four TGCs products have similar spectral characteristics and their maximum RRS wavelengths are all located at 291 nm. The quantitative determination ranges and the detection limits of the four TGCs are 0.0065-1.0 μg mL⁻¹ and 2.0 ng mL⁻¹ for CZD, 0.0070-1.1 μg mL⁻¹ and 2.2 ng mL⁻¹ for CTRX, 0.0090-1.6 μg mL⁻¹ and 2.7 ng mL⁻¹ for CPZ, and 0.014-2.2 μg mL⁻¹ and 4.2 ng mL⁻¹ for CFTM, respectively. The optimum conditions of the reactions and the effects of foreign substances are investigated, and the composition of ion-association complexes is discussed also. Based on the ion-association reaction, a highly sensitive, simple and rapid method has been proposed to the determination of TGCs.     

A study on the sizes and concentrations of gold nanoparticles by spectra of absorption, resonance Rayleigh scattering and resonance non-linear scattering

Liquid phase gold nanoparticles with different diameters and colors can be prepared using sodium citrate reduction method by controlling the amounts of sodium citrate. The mean diameters of gold nanoparticles are measured by transmission electron microscope (TEM). Gold nanoparticles with different sizes have specific absorption spectra. When the diameters of nanoparticles is between 12 and 41 nm, the maximum absorption peaks locate at 520-530 nm and there are red shifts gradually with the increase of diameters of gold nanoparticles. And when the size of gold nanoparticle is constant, the absorbance is proportional to the concentration of gold. Obvious resonance Rayleigh scattering (RRS) and the resonance non-linear scattering such as second-order scattering (SOS) and frequency-doubling scattering (FDS) appear at the same time as well, and the maximum scattering peaks are located at 286 nm (RRS), 480 nm (SOS) and 310 nm (FDS), respectively. When the concentration of gold is constant, absorbance and the intensities of RRS, SOS and FDS (I(RRS), I(SOS) and I(FDS)) have linear relationships with the diameters of gold nanoparticles. When the diameter of gold nanoparticle is constant, the absorbance and I(RRS), I(SOS), I(FDS) are directly proportional to the concentrations of gold nanoparticles. Therefore, it is very useful for studying the liquid phase gold nanoparticles by investigating the absorption, RRS, SOS and FDS spectra.     

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

In a weakly acid medium, some aminoglycoside antibiotics, such as kanamycin (KANA), gentamicin (GEN), tobramycin (TOB), and neomycin (NEO), or acid bisazo dye pontamine sky blue (PSB) can only produce very weak resonance Rayleigh scattering (RRS) signals. However, when the two agents react with each other to form the ion association complexes, the RRS intensity can be enhanced greatly and a new RRS spectrum and a significant enhancement of the RRS intensity in the wavelength range 350-600 nm can be observed. The maximum scattering peak is at 580 nm. There is a linear relationship between the RRS intensity and the antibiotic concentration in the range 0.01-6.0 microg mL(-1) at 580 nm. This RRS method has therefore been developed for the determination of trace levels of aminoglycoside antibiotics. The detection limits (3 sigma) of the four antibiotics, whose order of sensitivity is KANA>NEO>TOB>GEN, are 5.8-6.9 ng mL(-1). This method has a good selectivity and has been successfully applied to the quick determination of antibiotics not only for injections and ear drops, but clinic serum samples as well. In addition, quantum chemistry-based analysis of the reaction mechanism, the factors influencing the RRS spectra, and the reasons for the enhancement of RRS are discussed.     

Absorption,fluorescence and resonance Rayleigh scattering spectral characteristics of interaction of gold nanoparticle with safranine T

Gold nanoparticle is an important nanomaterial and has been investigated widely owing to its special physical and chemical property[1―5]. In recent years it has been found that the multiple-component nano- structure assembly containing metal, semiconduct…     

Study on the interaction between diphenhydramine and erythrosin by absorption,fluorescence and resonance Rayleigh scattering spectra

In pH 4.5 Britton-Robinson(BR)buffer solution,erythrosin(ET)can react with diphenhydramine(DP)to form a 1:1 ion-association complex,which not only results in the change of the absorption spectra,but also results in the great enhancement of resonance Rayleigh scattering(RRS)and the quenching of fluorescence.Furthermore,a new RRS spectrum will appear,and the maximum RRS wavelength was located at about 580 nm.In this work,the spectral characteristics of the absorption,fluorescence and RRS,the optimum conditions of the reaction and the properties of an analytical chemistry were inves- tigated.A sensitive,simple and new method for the determination of DP by using erythrosin as a probe has been developed.The detection limits for DP were 0.0020μg/mL for RRS method,0.088μg/mL for absorption method and 0.094μg/mL for fluorophotometry.There was a linear relationship between the absorbance,RRS and fluorescence intensities and the drug concentration in the range of 0.0067-2.0, 0.29-6.4 and 0.31-3.2μg/mL,respectively.The effects of the interaction of diphenhydramine and erythrosin on the absorption,fluorescence and resonance Rayleigh scattering spectra were discussed. In light polarization experiment,the polarization of RRS at maximum wavelength was measured to be P =0.9779,and it revealed that the RRS spectrum of DP-ET complex consists mostly of resonance scat- tering and few resonance fluorescence.In this study,enthalpy of formation and mean polarizability were calculated by AM1 quantum chemistry method.In addition,the reaction mechanism and the rea- sons for the enhancement of scattering spectra and the energy transfer between absorption,fluores- cence and RRS were discussed.     

Absorption and Rayleigh scattering and resonance Rayleigh scattering spectra of [HgX<Subscript>2</Subscript>]<Subscript>n</Subscript> nanoparticles

The composition and existing species ot the reaction production ot Hg ana X (X= Cl, Br and I) under different conditions, and their absorption, Rayleigh scattering (RS) and resonance Rayleigh scattering (RRS) spectra have been studied. The results show that the products exist in the form of nanoparticles as [HgX2]n aggregates under suitable conditions, and their average diameters increase with the increase of X- diameters. The diameters of [HgCI2]n, [HgBr2]n and [Hgl2]n are less than 4 nm, equal to 9 nm and 70 nm respectively. There are bathchromic shifts gradually with the increase of X- diameters in their absorption spectra. The absorption bands of [HgCI2]n and [HgBr2]n locate at ultraviolet region. However, the absorption band of [Hgl2]n is obvious in visible light region. Among three particles, only [Hgl2]n exhibits a strong RRS and its scattering peak is at 580 nm. The main reasons leading to the enhancement of resonance scattering are the large size of nanoparticle, the formation of the interface     

Resonance Rayleigh scattering spectra for studying the interaction of anthracycline antineoplastic antibiotics with some anionic surfactants and their analytical applications

In pH 5.8 acidic medium, the anionic surfactants such as sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS) or sodium dodecyl sulfonate (SLS) can react with anthracycline antibiotics such as epirubicin (EPI), daunorubicin (DNR) or mitoxantrone (MXT) to form ion-association complexes, which lead to a great enhancement of resonance Rayleigh scattering (RRS) intensity and appearances of new RRS spectra. The maximum RRS peaks are situated at 313 nm for SDS-DNR and SDS-EPI system, 296 nm for SDS-MXT system. The linear ranges and detection limits for EPI, DNR and MXT are 0.26-20.0, 0.25-20.0, 0.14-10.0 and 0.074, 0.078, 0.042 μg mL⁻¹, respectively. In this paper, the characteristics of the absorption, fluorescence and RRS spectra of the reaction products are studied as well as the optimum reaction conditions and analytical chemistry properties. A sensitive, simple and rapid RRS method for the determination of anthracycline anticancer antibiotics has been developed.