| HPLC法同时测定石蒜中加兰他敏和石蒜碱 |
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| 引用本文： | 李霞,熊海蓉,蒋利华,文祝友,熊远福. HPLC法同时测定石蒜中加兰他敏和石蒜碱[J]. 应用化学, 2010, 27(11): 1362-1364. DOI: 10.3724/SP.J.1095.2010.00002 |
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| 作者姓名： | 李霞 熊海蓉 蒋利华 文祝友 熊远福 |
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| 作者单位： | (湖南农业大学1.理学院,2.分析测试中心,4.动物科技学院 长沙 410128;3.长沙环境保护职业技术学院 长沙) |
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| 摘 要： | 建立了高效液相色谱同时测定石蒜中加兰他敏和石蒜碱含量的方法。 采用phenomenex-C18色谱柱，以乙腈(A)-0.1%TFA水溶液(B)为流动相梯度洗脱，检测波长289 nm。 结果表明，加兰他敏和石蒜碱均在0.5～10 mg/L(r=0.9999)呈现良好的线性关系，最低检测限(S/N=3)分别为0.09和0.15 mg/L，平均加样回收率分别为99.53%和96.82%。 该方法简单、快速、准确，适合于石蒜中加兰他敏和石蒜碱的测定。
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| 关 键 词： | 高效液相色谱 石蒜 加兰他敏 石蒜碱 |
| 收稿时间： | 2010-01-04 |
| 修稿时间： | 2010-06-29 |
Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC |
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| LI Xia,XIONG Hai-Rong,JIANG Li-Hua,WEN Zhu-You,XIONG Yuan-Fu. Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC[J]. Chinese Journal of Applied Chemistry, 2010, 27(11): 1362-1364. DOI: 10.3724/SP.J.1095.2010.00002 |
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| Authors: | LI Xia XIONG Hai-Rong JIANG Li-Hua WEN Zhu-You XIONG Yuan-Fu |
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| Affiliation: | (1.College of Science;2.Center of Analysis and Testing;4.College of Animal Science and Technology,;Hunan Agricultural University,Changsha 410128;3.Changsha Environmental Protection College,Changsha) |
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| Abstract: | A high performance liquid chromatographic method was developed for simultaneous determining the contents of galanthamine and lycorine in Lycoris Herb. The chromatographic analysis was carried out on a phenomenex-C18 column. The mobile phase was acetonitrile(A)-0.1%trifluoroacetic(TFA)(B) with gradient elution. The detection wavelength was set at 289 nm. The calibration curves for galanthamine and lycorine showed good linearity in the range of 0.5～10 mg/L(r=0.9999). The detection limits were 0.09 mg/L for galanthamine and 0.15 mg/L for lycorine at the signal-to-noise ratio of 3, and the average recoveries were 99.53% and 96.82%, respectively. The method is simple, rapid, accurate and suitable for the determination of galanthamine and lycorine in Lycoris Herb. |
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| Keywords: | HPLC Lycoris Herb galanthamine lycorine |
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