Evaluation of the dual retention properties of stationary phases based on silica hydride: Perfluorinated bonded material |
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| Authors: | Joseph J. Pesek Maria T. Matyska Harshada Natekar |
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| Affiliation: | Department of Chemistry, San Jose State University, San Jose, USA |
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| Abstract: | The synthesis of a new perfluorinated stationary phase based on silica hydride using a hydrosilation reaction was investigated. The material was characterized by elemental analysis, diffuse reflectance infrared Fourier transform spectroscopy and 13C cross‐polarization magic‐angle spinning NMR spectroscopy. The retention properties of this new material were tested in the reversed‐phase and normal‐phase mode. Variable buffer strength experiments at two pH conditions for selected polar compounds were used to compare the new phase to hydrophilic interaction liquid chromatography retention. These results and previous data reported in the literature were used to postulate differences in the retention mechanism between hydrophilic interaction liquid chromatography and silica hydride‐based stationary phases. |
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| Keywords: | Aqueous normal‐phase chromatography Retention mechanisms Spectroscopic characterization Solvent composition studies |
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