Preparation of (2E)-3-(4'-Halophenyl)prop-2-enoyl Sulfachlorpyridazine Sodium Salts and Their Interaction with Bovine Serum Albumin by Fluorescence Spectroscopy |
| |
| Authors: | DU Chuan-rong LUO Xuan WEI Jin-rui HE Ting-ting ZHENG Xiao-yu LIN Cui-wu |
| |
| Affiliation: | School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, P. R. China |
| |
| Abstract: | Three (2E)-3-(4'-halophenyl)prop-2-enoyl sulfachlorpyridazine sodium salts(XPSCA) were synthesized. Their chemical structures were confirmed by 1H NMR and 13C NMR, electrospray ionization mass spectrometry (ESI-MS), and infrared(IR) spectroscopy. The interactions between XPSCA and bovine serum albumin(BSA) were investigated under imitated physiological condition by fluorescence quenching technique and UV-Vis absorption spectroscopy according to the Stern-Volmer equation. The results from the emission quenching at different temperatures indicate that the quenching mechanism of serum albumin by XPSCA was static quenching mechanism at low XPSCA concentrations or a combined quenching(static and dynamic) mechanism at higher XPSCA concentrations. At different temperatures, the binding constant and the binding sites of XPSCA with BSA were investigated, and the distances were evaluated according to Förster non-radiative resonance energy transfer theory. The thermodynamic parameters were calculated according to van't Hoff equation, which implies that both van der Waals interaction and hydrogen bond played major roles in stabilizing the XPSCA-BSA complexes, whereas hydrophobic interactions were secondary. Moreover, the conformational changes in BSA were analyzed by synchronous fluorescence spectra. |
| |
| Keywords: | Sulfachlorpyridazine sodium Bovine serum albumin Halo-cinnamic acid Fluorescence spectroscopy |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《高等学校化学研究》浏览原始摘要信息 |
|
点击此处可从《高等学校化学研究》下载全文 |
|
