Simultaneous determination of propofol and remifentanil in rat plasma by liquid chromatography–tandem mass spectrometry: application to preclinical pharmacokinetic drug–drug interaction analysis |
| |
| Authors: | Mohamed A. El Hamd Mitsuhiro Wada Rie Ikeda Shigeru Kawakami Naotaka Kuroda Kenichiro Nakashima |
| |
| Affiliation: | 1. Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagasaki, Japan |
| |
| Abstract: | Propofol (Pro) is an ultra‐short‐acting hypnotic agent used for general anesthesia that has no analgesic properties. Remifentanil (Rem) is an ultra‐short‐acting opioid administered concomitantly as an analgesic with Pro. To evaluate the pharmacokinetic interactions between Pro and Rem, we developed and validated a method combining high‐performance liquid chromatography with tandem mass spectrometry for simultaneous determination of Pro and Rem. The proposed method was successfully used to study the pharmacokinetic interactions of Pro and Rem coadministered to rats. Copyright © 2014 John Wiley & Sons, Ltd. |
| |
| Keywords: | propofol remifentanil LC‐MS/MS rat plasma pharmacokinetic interactions |
|
