Methanol Perturbing Modeling Cell Membranes Investigated using Linear and Nonlinear Vibrational Spectroscopy (cited: 3) |
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| Authors: | Kangzhen Tian Hongchun Li Shuji Ye |
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| Affiliation: | 1.Department of Physics, University of Science and Technology of China, Hefei 230026, China2.Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China3.Department of Chemical Physics, University of Science and Technology of China, Hefei 230026, China;Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Tech-nology of China, Hefei 230026, China |
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| Abstract: | Cell membranes play a crucial role in many biological functions of cells. A small change in the composition of cell membranes can strongly influence the functions of membrane-associated proteins, such as ion and water channels, and thus mediate the chemical andphysical balance in cells. Such composition change could originate from the introduction of short-chain alcohols, or other anesthetics into membranes. In this work, we have applied sum frequency generation vibrational spectroscopy (SFG-VS), supplemented by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), to investigate interaction between methanol and 1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine (d54-DMPC) lipid bilayers. Lipid's hydrocarbon interior is deuterated while its head group is hydrogenated. At the same time, CH3 symmetric stretch from methanol and lipid head amine group has different frequency, thus we can distinguish the behaviors of methanol, lipid head amine group, and lipid hydrocarbon interior. Based on the spectral feature of the bending mode of the water molecules replaced by methanol, we determined that the methanol molecules are intercalated into the region between amine and phosphate groups at the lipid hydrophilic head. The dipole of CH3 groups of methanol and lipid head, and the water O-H all adopt the same orientation directions. The introduction of methanol into the lipid hydrophilic head group can strongly perturb the entire length of the alkyl chains, resulting that the signals of CD3 and CD3 groups from both leaflets can not cancel each other. |
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| Keywords: | Sum frequency generation vibrational spectroscopy Cell membrane Methanol Interaction Mechanism |
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