Rapid quick,easy, cheap,effective, rugged,and safe extraction with novel phospholipid cleanup: A streamlined ultra high performance liquid chromatography with ultraviolet detection approach for screening polycyclic aromatic hydrocarbons in avian blood cells and plasma |
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| Authors: | Anthony A. Provatas Alexander V. Yevdokimov Cory A. King Emma L. Gatley James D. Stuart David C. Evers Christopher R. Perkins |
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| Affiliation: | 1. Center for Environmental Sciences and Engineering, University of Connecticut, Storrs, CT, USA;2. Department of Chemistry, University of Rhode Island, Kingston, RI, USA;3. Department of Chemistry, University of Connecticut, Storrs, CT, USA;4. Biodiversity Research Institute, Portland, ME, USA |
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| Abstract: | A streamlined method has been developed for the isolation and analysis of polycyclic aromatic hydrocarbons in avian blood cells and plasma utilizing quick, easy, cheap, effective, rugged, and safe extraction in combination with novel phospholipid cleanup technology. A variety of traditional extraction and cleanup techniques have been employed in the preparation and analysis of polycyclic aromatic hydrocarbonsin a variety of matrices; liquid–liquid partitioning, solid‐phase extractions, gel permeation chromatography, and column chromatography are all effective techniques, however they are laborious and time consuming processes that require large amounts of solvent. Using quick, easy, cheap, effective, rugged, and safe extraction coupled with phospholipid cleanup, samples can be quickly screened while maintaining high throughput and sensitivity. With a liquid chromatography approach, analysis times may be kept short at 16 min while maintaining high analyte recovery. Recoveries in quality control samples ranged from 70 to 109%, with average surrogate recoveries of 80.6 ± 1.10%. The result of using a quick, easy, cheap, effective, rugged, and safe extraction approach in conjunction with phospholipid cleanup is a methodology that significantly reduces sample preparation time and solvent use while maintaining high sensitivity and reproducibility. |
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| Keywords: | Blood cells Polycyclic aromatic hydrocarbons Phospholipid cleanup / Quick easy cheap effective rugged safe Ultra high performance liquid chromatography |
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