High‐performance liquid chromatography with photodiode array detection and chemometrics method for the analysis of multiple components in the traditional Chinese medicine Shuanghuanglian oral liquid |
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| Authors: | Bao Qiong Li Jing Chen Jiao Jiao Li Xue Wang Hong Lin Zhai Xiao Yun Zhang |
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| Affiliation: | College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, P.R. China |
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| Abstract: | Shuanghuanlian oral liquid, a traditional Chinese medicine preparation, is a mixture of three herbs (Flos Lonicerae, Radix Scutellariae and Fructus Forsythiae). In this study, the quantitative analysis of three main active compounds, chlorogenic acid, forsythin and baicalin in samples from different manufacturers was performed rapidly by high‐performance liquid chromatography coupled with photodiode array detection followed by Contour Projection coupled to stepwise regression treatment of the obtained three‐dimensional spectra in which the partial overlap between adjacent target components existed. The method was validated for linearity (R>0.9940), precision (RSD<1.25%), recovery (92.20–102.50%), limit of detection (0.01–0.02 μg/mL) and limit of quantification (0.03–0.07 μg/mL). The results indicated that the combination of the three‐dimensional spectra of traditional Chinese medicine and Contour Projection‐stepwise regression offered an accurate, simple, low‐cost and eco‐friendly way for the rapid quantitative analysis of Shuanghuanlian oral liquid samples. |
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| Keywords: | Chemometrics Contour projection High‐performance liquid chromatography Quantitative analysis Three‐dimensional spectroscopy |
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