Screening of cannabinoids in industrial‐grade hemp using two‐dimensional liquid chromatography coupled with acidic potassium permanganate chemiluminescence detection |
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| Authors: | Jessica Pandohee Brendan J. Holland Bingshan Li Takuya Tsuzuki Paul G. Stevenson Neil W. Barnett James R. Pearson Oliver A.H. Jones Xavier A. Conlan |
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| Affiliation: | 1. School of Applied Sciences, RMIT University, Melbourne, Victoria, Australia;2. Centre for Chemistry and Biotechnology, School of Life and Environmental Sciences, Deakin University, Geelong, Victoria, Australia;3. Institute for Frontier Materials, Deakin University, Geelong, Victoria, Australia;4. Research School of Engineering, Australian National University, Canberra, Australia;5. Victoria Police Forensic Services Department, Macleod, Victoria, Australia |
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| Abstract: | Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two‐dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC‐C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices. |
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| Keywords: | Cannabis Offline chromatography Profiling 2D chromatography |
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