| 具有甲状腺素脱碘酶活性的抗体酶的化学修饰 |
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| 引用本文： | 孙立伟,刘立岩,赵大庆. 具有甲状腺素脱碘酶活性的抗体酶的化学修饰[J]. 高等学校化学学报, 2003, 24(4): 588-590 |
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| 作者姓名： | 孙立伟 刘立岩 赵大庆 |
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| 作者单位： | 中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春,130022;中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春,130022;中国科学院长春应用化学研究所稀土化学与物理开放实验室,长春,130022 |
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| 基金项目： | 国家自然科学基金重大项目 (批准号 :2 9890 2 80 ) |
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| 摘 要： | 甲状腺素脱碘酶是一种膜硒蛋白[1], 它能够将甲状腺素降解为不同产物, 并对甲状腺素的生理功能起调控作用. 但是, 甲状腺素脱碘酶极易变性失活, 到目前为止仍未得到纯酶[2]. 近来, 此酶的人工模拟工作逐渐成为热点. 我们小组[3]首次以3,5,3′,5′-四碘甲状腺原氨酸五水钠盐(T4)为半抗原, 采用单克隆抗体技术和苯甲基磺酰氟及硒氢化钠修饰的方法, 成功地制备了具有甲状腺素脱碘酶活性的抗体酶.
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| 关 键 词： | 必需基团 化学修饰 抗体酶 |
| 文章编号： | 0251-0790(2003)04-0588-03 |
| 收稿时间： | 2002-04-16 |
Chemical Modification of Abzyme with Deiodinase Activity |
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| SUN Li Wei,LIU Li Yan,ZHAO Da Qing. Chemical Modification of Abzyme with Deiodinase Activity[J]. Chemical Research In Chinese Universities, 2003, 24(4): 588-590 |
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| Authors: | SUN Li Wei LIU Li Yan ZHAO Da Qing |
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| Affiliation: | Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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| Abstract: | The abzyme with deiodinase activity was modified with diethyl pyrocarbonate(DEPC) and carbodiimide(EDC) respectively. The modified abzymes were dialyzed and their activities were compared with that of the native abzyme. The results indicate that the histidine residue is one of the essential groups in the abzyme, and the modification causes the catalytic activity a loss of 70%. The modification of the residues containing carboxyl group with EDC leads to a loss of 50% activity, indicating that acid amino residues play important roles in the catalytic reaction. |
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| Keywords: | Essential groups Chemical modification Abzyme |
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