| 12C6+离子辐照对阿维链霉菌代谢效应研究 |
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| 引用本文： | 王曙阳,薄永恒,王丽华,陈积红,李文建,梁剑平,刘敬. 12C6+离子辐照对阿维链霉菌代谢效应研究[J]. 原子核物理评论, 2013, 30(2): 195-200. DOI: 10.11804/NuclPhysRev.30.02.195 |
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| 作者姓名： | 王曙阳 薄永恒 王丽华 陈积红 李文建 梁剑平 刘敬 |
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| 作者单位： | 中国科学院近代物理研究所,甘肃 兰州 730000; |
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| 摘 要： | 对12C6+ 离子辐照诱变高产阿维链霉菌株ZJAV-Y1-203 和原始菌株ZJAV-A-1 的摇瓶发酵pH 值、菌体浓度、碳源和氮源代谢进行了测定，研究了12C6+ 辐照对阿维链霉菌的代谢效应。在发酵前期(48h)，原始菌株发酵液pH 值低于突变菌株；在发酵96~196 h，诱变高产菌株ZJAV-Y1-203 繁殖快，生长旺盛，N的利用率高；菌体浓度大于原始菌株的浓度，且发酵液的pH 稳定，菌体处于代谢相对更稳定期；在发酵144~240 h，诱变高产菌株ZJAV-Y1-203 对糖源消耗低于原始菌株ZJAV-A-1。这些结果表明，12C6+ 离子辐照对阿维链霉菌代谢影响有利于阿维菌素合成。pH value, mycelium concentration, carbon source and nitrogen metabolism in flask fermentation of the mutant high-producing strain ZJAV-Y1-203 and the original strain ZJAV-A1 have been investigated, in order to show the metabolic effect of avermitilis irradiated by ion beam of 12C6. In early stage (48 h) of the fermentation, pH value of the original fermentation was lower than that of the mutant strains. In 96~196 h of fermentation, the nitrogen utilization in the strains ZJAV-Y1-203 was higher than that in the original strains, its reproductive was fast, and its growing was vigorous. The mycelium concentration of ZJAV-Y1-203 was greater than the original strain, and the pH value of fermentation were stable, so its metabolism was relatively more stable. In 144240 h of fermentation, the strain ZJAV-Y1-203 on sugar consumption was less than the original strains. The effect of 12C6 ion irradiation on metabolism of Streptomyces avermitilis is conducive to the synthesis of avermectin.
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| 关 键 词： | pH 菌体浓度 糖含量 氮含量 代谢效应 |
| 收稿时间： | 1900-01-01 |
Metabolic Effect of Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+ |
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| WANG Shuyang,BO Yongheng,WANG Lihua,CHE Jihong,LI Wenjian,LIANG Jianping,LIU Jing. Metabolic Effect of Streptomyces Avermitilis Irradiated by Ion Beam of 12C6+[J]. Nuclear Physics Review, 2013, 30(2): 195-200. DOI: 10.11804/NuclPhysRev.30.02.195 |
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| Authors: | WANG Shuyang BO Yongheng WANG Lihua CHE Jihong LI Wenjian LIANG Jianping LIU Jing |
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| Affiliation: | Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;Gansu Agriculture University, Lanzhou 730070, China; |
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| Abstract: | pH value, mycelium concentration, carbon source and nitrogen metabolism in flask fermentation of the mutant high-producing strain ZJAV-Y1-203 and the original strain ZJAV-A1 have been investigated, in order to show the metabolic effect of avermitilis irradiated by ion beam of 12C6. In early stage (48 h) of the fermentation, pH value of the original fermentation was lower than that of the mutant strains. In 96~196 h of fermentation, the nitrogen utilization in the strains ZJAV-Y1-203 was higher than that in the original strains, its reproductive was fast, and its growing was vigorous. The mycelium concentration of ZJAV-Y1-203 was greater than the original strain, and the pH value of fermentation were stable, so its metabolism was relatively more stable. In 144240 h of fermentation, the strain ZJAV-Y1-203 on sugar consumption was less than the original strains. The effect of 12C6 ion irradiation on metabolism of Streptomyces avermitilis is conducive to the synthesis of avermectin. |
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