| 自组装DNA吸附取向的表面增强拉曼光谱法研究 |
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| 引用本文： | 董丽琴,周剑章,吴玲玲,董平,林仲华. 自组装DNA吸附取向的表面增强拉曼光谱法研究[J]. 高等学校化学学报, 2002, 23(12): 2303-2308 |
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| 作者姓名： | 董丽琴 周剑章 吴玲玲 董平 林仲华 |
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| 作者单位： | 厦门大学固体表面物理化学国家重点实验室, 厦门大学化学系, 物理化学研究所, 厦门361005 |
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| 基金项目： | 国家自然科学基金 (批准号 :2 980 30 0 7),教育部科技研究重点项目 (批准号:99177),教育部博士学科点基金资助 |
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| 摘 要： | 对金基体上自组装寡聚核苷酸探针杂交前后进行电化学非现场及现场表面增强拉曼光谱(SERS)研究.非现场SERS研究表明,杂交形成的dsDNA在基体表面以A型和B型两种构象同时存在,杂交过程可能伴随DNA链在基体表面吸附取向的变化.根据现场SERS研究结果可知,ssDNA及dsDNA的大多数SERS谱带强度随电极电位正移而降低,尤其是归属于碱基A的两种面外振动模式,谱带变化更为明显.利用SERS表面选择定则判断出随着电极电位由负向正变化,ssDNA及dsDNA螺旋吸附取向由垂直吸附向平躺吸附于金基体表面变化.
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| 关 键 词： | 寡聚核苷酸探针 杂交 表面增强拉曼光谱(SERS) 吸附取向 |
| 文章编号： | 0251-0790(2002)12-2303-06 |
| 收稿时间： | 2001-10-10 |
Surface Enhanced Raman Scattering(SERS) Studies on Adsorption Orientation on Self-assembled DNA |
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| DONG Li-Qin,ZHOU Jian-Zhang,WU Ling-Ling,DONG Ping,LIN Zhong-Hua. Surface Enhanced Raman Scattering(SERS) Studies on Adsorption Orientation on Self-assembled DNA[J]. Chemical Research In Chinese Universities, 2002, 23(12): 2303-2308 |
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| Authors: | DONG Li-Qin ZHOU Jian-Zhang WU Ling-Ling DONG Ping LIN Zhong-Hua |
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| Affiliation: | State Key Laboratory for Physical Chemistry of the Solid Surface, Department of Chemistry, Institute of Physical Chemistry, Xiamen University, Xiamen 361005, China |
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| Abstract: | A detailed study is conducted for the electrochemical ex situ and in situ surface enhanced Raman scattering(SERS) studies of oligonucleotide probe self-assembled monolayer on gold substrate before and after hybridization. Ex situ SERS results indicate that dsDNA molecules on gold substrate are not only in B-form but also in A-form helices and that the DNA molecules have the adsorption orientation change after the hybridization. According to the in situ SERS results, the intensities of most of the SERS bands of {ssDNA} and dsDNA decrease substantially toward more positive potentials, especially for two out-of-plane vibration modes of adenine. By utilizing SERS "surface selection rules", it can be concluded that as the potential is progressively changed from negative to more positive potentials, the adsorption orientation of {ssDNA} and dsDNA changes from standing straight up to lying flat down on the gold substrate. |
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| Keywords: | Oligonucleotide probe Hybridization Surface Enhanced Raman Scattering(SERS) Adsorptive orientation |
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