| 全氟辛烷磺酸与蛋白质体系的共振光散射光谱及其分析应用 |
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| 引用本文： | 吴飞,朱进,谭克俊. 全氟辛烷磺酸与蛋白质体系的共振光散射光谱及其分析应用[J]. 应用化学, 2012, 29(8): 969-973. DOI: 10.3724/SP.J.1095.2012.00387 |
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| 作者姓名： | 吴飞 朱进 谭克俊 |
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| 作者单位： | (三峡库区生态环境教育部重点实验室,西南大学化学化工学院 重庆 400715) |
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| 基金项目： | 国家自然科学基金(20877063);西南大学大型仪器设备开放基金资助项目 |
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| 摘 要： | 研究了牛血清白蛋白(BSA)与全氟辛烷磺酸(PFOS)相互作用的共振光散射(RLS)光谱,建立了PFOS的共振光散射分析方法。 在pH值为4.1的BR缓冲溶液中,全氟辛烷磺酸根阴离子与质子化的BSA通过静电引力和疏水作用形成离子缔合物,引起共振光散射强度(IRLS)显著增强,最大散射波长位于285.0 nm处,增强的散射信号强度与PFOS浓度在0.2～25.0 μmol/L范围内呈线性关系,据此建立了测定PFOS的光散射分析方法,检出限为20.0 nmol/L。 讨论了体系的最佳反应条件及外来物质的干扰,并探讨了反应机理。 建立的共振光散射法用于环境水样中PFOS的测定,RSD≤4.4%。
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| 关 键 词： | 牛血清白蛋白 全氟辛烷磺酸 共振光散射 |
| 收稿时间： | 2011-09-02 |
| 修稿时间： | 2011-10-14 |
Resonance Light Scattering Spectra of Perfluorooctane Sulfonate-Protein System and Its Analytical Application |
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| WU Fei,ZHU Jin,TAN Kejun. Resonance Light Scattering Spectra of Perfluorooctane Sulfonate-Protein System and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, 2012, 29(8): 969-973. DOI: 10.3724/SP.J.1095.2012.00387 |
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| Authors: | WU Fei ZHU Jin TAN Kejun |
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| Affiliation: | (Key Laboratory of Eco-Environments of Three Gorges Reservoir Region under Ministry of Education of PRC,;College of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China) |
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| Abstract: | The interaction of bovine serum albumin(BSA) with perfluorooctane sulfonate(PFOS) was investigated with resonance light scattering(RLS) spectroscopy.A RLS method for the determination of PFOS was established.In pH=4.1 Britton-Robinson buffer solution,perfluorooctane sulfonate anions reacted with the protonated BSA by electrostatic forces and hydrophobic interactions to form ion-association complexes and resulted in greatly enhanced resonance light scattering signals characterized by a peak at 285.0 nm.The RLS intensity of system was proportional to the content of PFOS in the range of 0.2～25.0 μmol/L,and the limit of detection was 20.0 nmol/L.The optimum reaction conditions and interference of foreign substances were investigated.This RLS method was applied to the determination of PFOS in environmental samples with RSD≤4.4%. |
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| Keywords: | bovine serum albumin perfluorooctane sulfonate resonance light scattering |
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