Quality Assessment of Two Spectrophotometric Procedures for Polyphenol Determination and Application in Moltkia petraea Species |
| |
| Authors: | Jadranka Vuković Rodríguez Renata Jurišić Grubešić Dario Kremer Virna Kokot |
| |
| Affiliation: | 1. Department of Pharmaceutical Analysis, Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovacica 1, HR‐10000 Zagreb, Croatia, Tel: +385?1?4818288;2. Fax: +385?1?4920089;3. Department of Pharmaceutical Botany with Fran Ku?an Botanical Garden, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 37, HR‐10000 Zagreb, Croatia |
| |
| Abstract: | A comparison study of quality of two spectrophotometric procedures: Schneider’s (T‐Sch) and 4‐aminoantipyrine (T‐AAP) procedures for total polyphenols determination was carried out using informative prevalidation strategy. Good quality of the measurements at the lower analyte level, excellent resolution of signals, homogenous data material, ideal linear calibration and analytical evaluation functions, very low limit of quantitation (0.13 μg/mL), and high precision (from ±0.60 % to ±3.43 %) and accuracy (from ?4.26 % to +4.00 %) confirmed the high quality of the T‐Sch procedure. Although favorable performance characteristics of T‐AAP procedure were obtained, the signal resolution, sensitivity, precision and accuracy are somewhat lower than in T‐Sch procedure. Both prevalidated procedures were successfully applied to determine total polyphenols in leaves, stems, and flowers in three populations of endemic Illyric? Balkan species Moltkia petraea (Tratt.) Griseb. growing in Croatia. Total concentrations of polyphenols obtained by T‐Sch procedure in all plant organs of all populations were somewhat lower than determined by T‐AAP procedure. However, the relative abundance of polyphenols obtained by both methods is the same. Prevalidation results demonstrated the reliability of the T‐AAP and T‐Sch procedures as valuable tools in polyphenols analysis and their application provided new information regarding phytochemical characterization of endemic M. petraea. |
| |
| Keywords: | Total polyphenols Prevalidation Quality control Moltkia petraea species |
|
|
