On the electron transfer through Geobacter sulfurreducens PilA protein |
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| Authors: | Nikolai Lebedev Syed Mahmud Igor Griva Anders Blom Leonard M. Tender |
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| Affiliation: | 1. Center for Bio‐Molecular Science and Engineering, Naval Research Laboratory, Washington, DC;2. Department of Mathematical Sciences and Computational Material Science Center George Mason University, Fairfax, Virginia;3. QuantumWise A/S, Copenhagen, Denmark |
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| Abstract: | Geobacter sulfurreducens pili composed of the Type IV pili structural peptide PilA have been implicated as efficient electronic conductors. Though investigated experimentally, no detailed theoretical studies have been performed to date that provide quantitative estimation of the transmission spectrum, electron transfer (ET) paths, efficiency of current generation, and other factors needed for understanding possible mechanisms of conductivity. In the present work, we calculate from first principles the possibilities of electron tunneling through 3 PilA fragments which structure was identified recently by NMR. The results indicate that positively charged amino acids, arginines and lysines form electrostatic traps in the middle of the peptide preventing ET at low bias voltages (<～6 V). At higher biases the traps are filled with electrons making possible sequential electron tunneling through the central part of the protein. In addition, leucines and phenylalanines form ET loops facilitating electron stabilization within the protein and sequential ET. Our results indicate that ET through the PilA protein cannot occur by coherent ET, but suggest a sequential (incoherent) mechanism. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2015 , 53, 1706–1717 |
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| Keywords: | conductive pili Geobacter sulfurreducens electroactive biofilm protein conductance molecular electronics |
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