RAFT of sulfobetaine for modifying poly(glycidyl methacrylate) microspheres to reduce nonspecific protein adsorption |
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| Authors: | Jana Koubková Hana Macková Vladimír Proks Miroslava Trchová Jiří Brus Daniel Horák |
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| Affiliation: | 1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Czech Republic;2. Institute of Chemistry and Technology of Macromolecular Materials, Faculty of Chemical Technology, University of Pardubice, Czech Republic |
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| Abstract: | The minimization of nonspecific protein adsorption is a crucial step in the development of bioseparation processes, immunoassays, and affinity diagnostics. Among the numerous biomaterials, polyzwitterions are known to effectively suppress protein and cell adhesion. This article describes the formation of monodisperse polymer microspheres coated with polysulfobetaine with the aim to limit nonspecific adsorption of bovine serum albumin (BSA) as a model protein. In this process, 2‐μm poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization. To render the microspheres hydrophilic and biocompatible, [3‐(methacryloylamino)propyl]dimethyl(3‐sulfopropyl)ammonium hydroxide (MPDSAH) was grafted from the surface by reversible addition‐fragmentation chain transfer (RAFT) polymerization. Elemental analysis of the modified microspheres revealed up to 20 wt % of poly{[3‐(methacryloylamino)propyl]dimethyl(3‐sulfopropyl)ammonimum hydroxide} (PMPDSAH). The microspheres were characterized in terms of particle size, morphology, and zeta potential. The amount of BSA nonspecifically adsorbed on the PMPDSAH‐modified microspheres decreased to half of that captured on the unmodified PGMA microspheres. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015 , 53, 2273–2284 |
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| Keywords: | adsorption grafting hydrophilic polymers microspheres polysulfobetaine reversible addition fragmentation chain transfer (RAFT) synthesis |
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