Selective Antimicrobial and Antibiofilm Disrupting Properties of Functionalized Diamond Nanoparticles Against Escherichia coli and Staphylococcus aureus |
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| Authors: | Manakamana Khanal Viktoria Raks Rahaf Issa Volodymyr Chernyshenko Alexandre Barras Jose M. Garcia Fernandez Lyuba I. Mikhalovska Volodymyr Turcheniuk Vladimir Zaitsev Rabah Boukherroub Aloysius Siriwardena Ian R. Cooper Peter J. Cragg Sabine Szunerits |
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| Affiliation: | 1. Institute of Electronics, Microelectronics and Nanotechnology (IEMN),UMR‐CNRS 8520, Université Lille 1, Cité Scientifique, Villeneuve d'Ascq, France;2. Taras Shevchenko University, Kiev, Ukraine;3. School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK;4. O. V. Palladin National Institute of Biochemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine;5. Investigaciones Químicas (IIQ), CSIC – Universidad de Sevilla avda, Sevilla, Spain;6. Chemistry Department, Pontifical Catholic University of Rio de Janeiro, Rua Marques de Sao Vicente, Rio de Janeiro, Brazil;7. Laboratoire de Glycochimie des Antimicrobiens et des Agroressources (LG2A), (FRE 3517‐CNRS), Université de Picardie Jules Vernes, Amiens, France |
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| Abstract: | Diamond nanoparticles (NDs) have demonstrated great promise as useful materials in a variety of biomedical settings. In this paper, the antimicrobial and antibiofilm activities of variously functionalized NDs against two common bacterial targets Gram‐negative bacterium Escherichia coli and Gram‐positive bacterium Staphylococcus aureus are compared. Hydroxylated (ND‐OH), aminated (ND‐NH2), carboxylated (ND‐COOH), mannose (ND‐Mannose), tri‐thiomannoside (ND‐Man3), or tri‐thiolactoside (ND‐Lac3)‐modified NDs are fabricated and evaluated in the present work. Of these, the mannose‐modified NDs are found to interfere most strongly with the survival of S. aureus, but not to influence the growth of E. coli. In contrast, particles featuring lactosyl units have the opposite effect on S. aureus growth. Sugar‐functionalized NPs reported to display antibacterial effects are rare. Only ND‐COOH particles are seen to have any effect on the growth profile of E. coli, but the effects are moderate. On the other hand, both ND‐NH2 and ND‐COOH are found to inhibit E. coli‐induced biofilm formation at levels comparable to the known E. coli biofilm disruptor, ampicillin (albeit at concentrations of 100 μg mL?1). However, none of the modified particles examined here reveal any significant activity as disruptors of S. aureus‐induced biofilm formation even at the highest concentrations studied. |
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| Keywords: | antimicrobial activity biofilm inhibition diamond nanoparticles functionalization surface termination |
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