A Photoreactive G‐Quadruplex Ligand Triggered by Green Light |
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| Authors: | Dr. Matteo Nadai Dr. Filippo Doria Dr. Luca Germani Prof. Sara N. Richter Prof. Mauro Freccero |
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| Affiliation: | 1. Department of Molecular Medicine, University of Padua, Via Gabelli 63, 35121 Padua (Italy);2. Department of Chemistry, University of Pavia, Via Taramelli 10, 27100 Pavia (Italy) |
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| Abstract: | A photoreactive molecular dye targeting the G‐quadruplex nucleic acid (G4) of the human telomeric sequence Tel22, and several mutated analogues, was activated by green light (λ=532 nm). Highly selective covalent modification of G4 versus single‐stranded and double‐stranded DNA was achieved with efficiency up to 64 %. The phenoxyl radical was generated and detected by laser‐flash photolysis as a reactive intermediate that targeted loop thymine residues. These insights may suggest a non‐invasive tool for selective nucleic acid tagging and “pull‐down” cellular applications. |
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| Keywords: | covalent targeting DNA damage G‐quadruplexes nucleic acids photochemistry |
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