Au Nanoparticle-DNAzyme Dual Catalyst System for Sensitively Colorimetric Detection of Thrombin |
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| Authors: | RAO Xin-yi ZHANG Jia-jia CUI Jing HU Ying LIU Ting CHAI Jing-feng CHENG Gui-fang HE Pin-gang FANG Yu-zhi |
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| Affiliation: | 1. Department of Chemistry, East China Normal University, Shanghai 200241, P. R. China;
2. Shanghai International Airport Co., Ltd., Shanghai 201202, P. R. China |
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| Abstract: | A novel colorimetric aptasensor was developed for thrombin detection with high sensitivity and specificity. The assay takes the advantage of Au nanoparticles-DNAzyme as a dual catalytic system for signal amplification. Au nanparticles were modified with peroxidase mimicking DNAzyme sequence as well as thrombin binding aptamer. And then the thrombin binding aptamer hybridized with its complementary sequence which was immobilized on the surface of the magnetic nanoparticles to construct the colorimetric aptasensor. In the presence of thrombin, the target-induced displacement takes place, resulting in the dissociation of the aptasensor. The DNAzyme functioned Au NPs are released due to the combine ability of thrombin binding aptamer with thrombin. The released Au NPs are capable of catalyzing the colorless 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid(ABTS) conversion into a blue-green product by H2O2-mediated oxidation, thus can amplify the colorimetric readout signals of thrombin detection. Such a device can serve as a novel selectivity sensor for thrombin with a detection limit of 0.6 nmol/L. |
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| Keywords: | Au nanoparticle Magnetic nanoparticle DNAzyme Aptamer Thrombin |
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