Cross‐Linked Aptamer–Lipid Micelles for Excellent Stability and Specificity in Target‐Cell Recognition |
| |
| Authors: | Xiaowei Li Dr. C. Adrian Figg Dr. Ruowen Wang Dr. Ying Jiang Dr. Yifan Lyu Hao Sun Dr. Yuan Liu Dr. Yanyue Wang Dr. I‐Ting Teng Dr. Weijia Hou Dr. Ren Cai Dr. Cheng Cui Long Li Xiaoshu Pan Prof. Brent S. Sumerlin Prof. Weihong Tan |
| |
| Affiliation: | 1. Center for Research at the Bio/Nano Interface, Department of Chemistry and Department of Physiology and Functional Genomics, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL, USA;2. George and Josephine Butler Polymer Research Laboratory, Center for Macromolecular Science and Engineering, Department of Chemistry, University of Florida, Gainesville, FL, USA;3. Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Life Sciences and College of Chemistry and Chemical Engineering, Aptamer Engineering, Center of Hunan Province, Hunan University, Changsha, Hunan, China;4. Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, College of Chemistry and Chemical Engineering, Shanghai, China |
| |
| Abstract: | The specific binding ability of DNA–lipid micelles (DLMs) can be increased by the introduction of an aptamer. However, supramolecular micellar structures based on self‐assemblies of amphiphilic DLMs are expected to demonstrate low stability when interacting with cell membranes under certain conditions, which could lead to a reduction in selectivity for targeting cancer cells. We herein report a straightforward cross‐linking strategy that relies on a methacrylamide branch to link aptamer and lipid segments. By an efficient photoinduced polymerization process, covalently linked aptamer–lipid units help stabilize the micelle structure and enhance aptamer probe stability, further improving the targeting ability of the resulting nanoassembly. Besides the development of a facile cross‐linking method, this study clarifies the relationship between aptamer–lipid concentration and the corresponding binding ability. |
| |
| Keywords: | aptamer– lipid micelles biostability cross-linking self-assembly specific cell recognition |
|
|
