Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces |
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| Authors: | Dr. Norifumi Kawakami Hiroki Kondo Yuki Matsuzawa Kaoru Hayasaka Erika Nasu Kenji Sasahara Dr. Ryoichi Arai Prof. Kenji Miyamoto |
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| Affiliation: | 1. Department of Bioscience and Informatics, Faculty of Science and Technology, Keio University, Yokohama, Kanagawa, Japan;2. Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan;3. Department of Supramolecular Complexes, Research Center for Fungal and Microbial Dynamism, Shinshu University, Minamiminowa, Nagano, Japan |
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| Abstract: | Protein‐based nanoparticles hold promise for a broad range of applications. Here, we report the production of a uniform anionic hollow protein nanoparticle, designated TIP60, which spontaneously assembles from a designed fusion protein subunit based on the geometric features of polyhedra. We show that TIP60 tolerates mutation and both its interior and exterior surfaces can be chemically modified. Moreover, TIP60 forms larger structures upon the addition of a cationic protein. Therefore, TIP60 can be used as a modifiable nano‐building block for further molecular assembly. |
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| Keywords: | cage proteins fusion protein nanoparticles protein design self-assembly |
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