Rapid Photoactuation of a DNA Nanostructure using an Internal Photocaged Trigger Strand |
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| Authors: | Dr. Minghui Liu Dr. Shuoxing Jiang Omar Loza Dr. Nour Eddine Fahmi Dr. Petr Šulc Dr. Nicholas Stephanopoulos |
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| Affiliation: | 1. Center for Molecular Design and Biomimetics, The Biodesign Institute, Arizona State University, Tempe, AZ, USA;2. Department of Physics, Arizona State University, Tempe, AZ, USA;3. School of Molecular Sciences, Arizona State University, Tempe, AZ, USA |
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| Abstract: | A reconfigurable DNA nano‐tweezer is reported that can be switched between a closed and open state with a brief pulse of UV light. In its initial state, the tweezer is held shut using a hairpin with a single‐stranded poly‐A loop. Also incorporated in the structure is a poly‐T trigger strand bearing seven photocaged residues. Upon illumination with 365 nm light, the cages are removed and the trigger strand hybridizes to the loop, opening the tweezer and increasing the distance between its arms from 4 to 18 nm. This intramolecular process is roughly 60 times faster than adding an external trigger strand, and provides a mechanism for the rapid interconversion of DNA nanostructures with light. |
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| Keywords: | biophysics DNA nanotechnology nanomachines photoswitches self-assembly |
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