Determination of swertianolin in rat plasma by LC‐MS/MS and its application to a pharmacokinetic study |
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| Authors: | Jun He Chengwang Tian Huizi Ouyang Tiwalade A. Adelakun Bin Yu Yanxu Chang Guixiang Pan Linghuo Jiang Xiumei Gao |
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| Affiliation: | 1. Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China;2. School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China;3. The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China |
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| Abstract: | A sensitive and rapid LC‐MS/MS method has been developed and validated for quantifying swertianolin in rat plasma using rutin as an internal standard (IS). Following liquid–liquid extraction with ethyl acetate, chromatographic separation for swertianolin was achieved on a C18 column with a gradient elution using 0.1% formic acid as mobile phase A and acetonitrile as mobile phase B at a flow rate of 0.3 mL/min. The detection was performed on a tandem mass spectrometer using multiple reaction monitoring via an electrospray ionization source and operating in the negative ionization mode. The optimized mass transition ion pairs (m/z) for quantitation were 435.1/272.0 for swertianolin and 609.2/300.1 for IS. The lower limit of quantitation was 0.5 ng/mL within a linear range of 0.5–500 ng/mL. Intra‐day and inter‐day precision was less than 6.8%. The accuracy was in the range of ?13.9 to 12.0%. The mean recovery of swertianolin was >66.7%. The proposed method was successfully applied in evaluating the pharmacokinetics of swertianolin after an oral dose of 50 mg/kg Swertia mussotii extract in rats. Copyright © 2014 John Wiley & Sons, Ltd. |
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| Keywords: | swertianolin LC‐MS/MS pharmacokinetics |
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