Interaction Between Cytochrome c and the Hapten 2,4-Dinitro-fluorobenzene by Electrospray Ionization Mass Spectrometry |
| |
| Authors: | Bo Wu Yan-qiu Chu Zhao-yun Dai Chuan-fan Ding¤ |
| |
| Affiliation: | {{each article.affiliations aff i}} {{if aff.addressEn && aff.addressEn != ""}} {{if aff.label && aff.label != "" && article.affiliations.length != 1}}{{@ aff.label}}.{{/if}}{{@ aff.addressEn}}{{/if}} {{/each}} |
| |
| Abstract: | Allergic contact dermatitis is a delayed hypersensitivity reaction, which results from skin exposure to low molecular weight chemicals such as haptens. To clarify the pathogenic mechanism, electrospray ionization mass spectrometry (ESI-MS) and hydrogen/deuterium (H/D) exchange, as well as UV spectroscopy, were applied to determine the interaction between the model protein cytochrome c (cyt c) and the hapten 2,4-dinitro-fluorobenzene (DNFB). The ESI-MS results demonstrate that the conformation of cyt c can change from native folded state into partially unfolded state with the increase of DNFB. The equilibrium state H/D exchange followed by ESI-MS further confirms the above results. UV spectroscopy indicates that the strongfield coordination between iron of heme (prosthetic group) and His18 or Met80 of cyt c is not obviously affected by the hapten. |
| |
| Keywords: | Interaction Cytochrome c 2 4-dinitro-fluorobenzene ESI-MS |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《化学物理学报(中文版)》浏览原始摘要信息 |
|
点击此处可从《化学物理学报(中文版)》下载免费的PDF全文 |
