| 基于液滴界面不稳定性的表面粗糙聚合物微球的制备及其细胞捕获应用 |
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| 引用本文： | 王月桐,商珞然,赵远锦. 基于液滴界面不稳定性的表面粗糙聚合物微球的制备及其细胞捕获应用[J]. 物理学报, 2020, 0(8): 172-179 |
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| 作者姓名： | 王月桐 商珞然 赵远锦 |
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| 作者单位： | 东南大学生物科学与医学工程学院;复旦大学生物医学研究院 |
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| 基金项目： | 国家自然科学基金(批准号:61927805,51522302);国家自然科学基金委员会-中国工程物理研究院联合基金(批准号:U1530260);江苏省自然科学基金(批准号:BE2018707);复旦大学和中山医院徐汇医院启动研究基金(批准号:JIH1340032)资助的课题。 |
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| 摘 要： | 具有不同组成和形态的聚合物颗粒近来受到越来越多的关注,它们的表面粗糙度显著影响着其理化性能,尤其在调节生物材料与生物系统间的相互作用中发挥着重要作用.本文设计了一种具有表面可调褶皱结构的聚苯乙烯微球.首先通过微流控装置产生尺寸均一的含有疏水聚合物和助表面活性剂的液滴.在有机溶剂的挥发过程中,不断收缩的液滴出现界面不稳定现象.表面面积自发增大,固化后得到表面具有褶皱的微球.研究结果表明,调节助表面活性剂的浓度以及溶剂挥发速率均可以有效调控微球表面粗糙程度.循环肿瘤细胞捕获实验表明,这种褶皱结构能明显增强细胞黏附力,提高细胞捕获量.以上这些特征表明这种表面褶皱微球将在生物医学分析领域具备良好的应用前景.
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| 关 键 词： | 界面不稳定性 微流控 表面褶皱 微球 细胞捕获 |
Surface-textured polymer microspheres generated through interfacial instabilities of microfluidic droplets for cell capture |
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| Wang Yue-Tong,Shang Luo-Ran,Zhao Yuan-Jin. Surface-textured polymer microspheres generated through interfacial instabilities of microfluidic droplets for cell capture[J]. Acta Physica Sinica, 2020, 0(8): 172-179 |
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| Authors: | Wang Yue-Tong Shang Luo-Ran Zhao Yuan-Jin |
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| Affiliation: | (State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering,Southeast University,Nanjing 210096,China;Zhongshan-Xuhui Hospital,Institutes of Biomedical Sciences,Fudan University,Shanghai 200032,China) |
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| Abstract: | Polymer microparticles with various compositions and morphologies have recently received much attention.Their surface-roughness significantly affects the physical and chemical properties, which especially counts in regulating the interaction between biological materials and living systems. In this paper, we design a polystyrene microsphere with controllable surface textures. At first, a microfluidic device is used to generate droplets with uniform size containing the hydrophobic polymer and a co-surfactant. During the volatilization of the organic solvent, the shrinking droplets appear to be unstable at the interface. Thus, the surface area increases spontaneously, and microspheres with wrinkles on the surface are obtained after being solidified. The results show that tuning the concentration of the co-surfactant and the rate of solvent evaporation can effectively regulate the surface roughness of the microspheres. Circulating tumor cell capture experiments reveal that this textured structure can facilitate the cell adhesion and increase the number of the captured cells. These features indicate that the coarse microspheres possess a promising application prospect in the field of biomedical analysis. |
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| Keywords: | interfacial instability microfluidics surface-texture microspheres cell capture |
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