| 微乳液和微乳液凝胶中脂肪酶催化的酯合成反应 |
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| 引用本文： | 周国伟,李干佐,李越中,徐健,王仁会,李传光. 微乳液和微乳液凝胶中脂肪酶催化的酯合成反应[J]. 化学学报, 2001, 59(3): 344-349 |
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| 作者姓名： | 周国伟 李干佐 李越中 徐健 王仁会 李传光 |
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| 作者单位： | 山东大学胶体与界面化学教育部重点实验室;山东大学微生物技术国家重点实验 室 |
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| 基金项目： | 国家自然科学基金(29903006)资助项目 |
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| 摘 要： | 在ACT/异辛烷/水形成的油包水微乳液中，研究了Candidalipolytical(CL)脂肪酶催化庚酸和庚醇的酯化反应，动力学研究表明反应符合乒乓(Ping-Pong)BiBi机制，两底物酸和醇均有抑制效应，并测定了反应的表观动力学常数，将CL脂肪酶固定于含明胶的微乳液凝胶(MBGs)中，可制得固定化脂肪酶，含酶的MBGs在非极性有机溶剂中可作为一种新的固相催化剂，并研究了MBGs在异辛烷中催化合成酯反应的性能，所制得的MBGs重复利用性和贮存稳定性都非常好。
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| 关 键 词： | 微乳 脂肪酶 酶催化 固定化酶 酯化 |
| 修稿时间： | 2000-07-04 |
Esterification reaction catalyzed by lipase in W/O microemulsion based organogels |
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| Zhou Guowei,Li Ganzuo,Li Yuezhong,Xu Jian,Wang Renhui,Li Chuanguang. Esterification reaction catalyzed by lipase in W/O microemulsion based organogels[J]. Acta Chimica Sinica, 2001, 59(3): 344-349 |
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| Authors: | Zhou Guowei Li Ganzuo Li Yuezhong Xu Jian Wang Renhui Li Chuanguang |
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| Abstract: | Esterification reactions of heptanoic acid with heptanol, catalyzed by Candida lypolytical (CL) lipase, was studied in water-in-oil microemulsions formed by water/bis-(2-ethylhexyl) sulfosuccinate sodium (AOT)/isooctane. Kinetic studies showed that the reaction follows a Ping-Pong Bi Bi mechanism with inhibition by both substrates. The values of all apparent kinetic parameters were determined. Lipase has also been immobilized in gelatin-containing AOT microemulsion-based organogels (MBGs) without any loss of catalytic activity. These lipase-containing MBGs prove to be novel solid-phase catalysts for use in apolar organic solvents and have good storage stability. These lipase-containing MBGs retained its higher activity after many runs esterification reactions. |
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| Keywords: | MICROEMUSION LIPASE ENZYME CATALYSIS FIXED ENZYME ESTERIFICATION |
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