An Efficient One‐Pot Four‐Segment Condensation Method for Protein Chemical Synthesis |
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| Authors: | Shan Tang Yan‐Yan Si Zhi‐Peng Wang Kun‐Rong Mei Xin Chen Jing‐Yuan Cheng Dr. Ji‐Shen Zheng Prof. Dr. Lei Liu |
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| Affiliation: | 1. Tsinghua‐Peking Center for Life Sciences, Department of Chemistry, Tsinghua University, Beijing 100084 (China);2. Tsinghua‐Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084 (China);3. High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031 (China) |
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| Abstract: | Successive peptide ligation using a one‐pot method can improve the efficiency of protein chemical synthesis. Although one‐pot three‐segment ligation has enjoyed widespread application, a robust method for one‐pot four‐segment ligation had to date remained undeveloped. Herein we report a new one‐pot multisegment peptide ligation method that can be used to condense up to four segments with operational simplicity and high efficiency. Its practicality is demonstrated by the one‐pot four‐segment synthesis of a plant protein, crambin, and a human chemokine, hCCL21. |
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| Keywords: | native chemical ligation peptides protecting groups protein chemical synthesis solid‐phase synthesis |
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