Simultaneous Capture,Detection, and Inactivation of Bacteria as Enabled by a Surface‐Enhanced Raman Scattering Multifunctional Chip |
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| Authors: | Dr. Houyu Wang Dr. Yanfeng Zhou Xiangxu Jiang Bin Sun Ying Zhu Hui Wang Dr. Yuanyuan Su Prof. Yao He |
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| Affiliation: | Institute of Functional Nano & Soft Materials (FUNSOM) and Collaborative Innovation Center of Suzhou Nanoscience and Technology (NANO‐CIC), Soochow University, Suzhou 215123 (China) |
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| Abstract: | Herein, we present a multifunctional chip based on surface‐enhanced Raman scattering (SERS) that effectively captures, discriminates, and inactivates pathogenic bacteria. The developed SERS chip is made of a silicon wafer decorated with silver nanoparticles and modified with 4‐mercaptophenylboronic acid (4‐MPBA). It was prepared in a straightforward manner by chemical reduction assisted by hydrogen fluoride etching, followed by the conjugation of 4‐MPBA through Ag? S bonds. The dominant merits of the fabricated SERS chip include excellent reproducibility with a relative standard deviation (RSD) value smaller than 11.0 %, adaptable bacterial‐capture efficiency (ca. 60 %) at low concentrations (500–2000 CFU mL?1), a low detection limit (down to a concentration of 1.0×102 cells mL?1), and high antibacterial activity (an antibacterial rate of ca. 97 %). The SERS chip enabled sensitive and specific discrimination of Escherichia coli and Staphylococcus aureus from human blood. |
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| Keywords: | bacteria multifunctional platforms silicon silver nanoparticles surface‐enhanced Raman scattering |
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