| 超高效液相色谱-串联质谱法同时测定粮食及肉制品中的24种工业染料 |
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| 引用本文： | 冯月超,贾丽,何亚荟,王建凤,刘艳,范筱京. 超高效液相色谱-串联质谱法同时测定粮食及肉制品中的24种工业染料[J]. 色谱, 2013, 31(10): 1021-1027. DOI: 10.3724/SP.J.1123.2013.03019 |
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| 作者姓名： | 冯月超 贾丽 何亚荟 王建凤 刘艳 范筱京 |
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| 作者单位： | 北京市理化分析测试中心, 北京市食品安全分析测试工程技术研究中心, 北京 100089 |
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| 基金项目： | 北京市科技专项项目(Z111106055311093). |
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| 摘 要： | 建立了测定粮食及肉制品中24种禁用工业染料的超高效液相色谱-串联质谱法。样品经甲醇、乙腈提取后,过WAX固相萃取柱净化。采用ACQUITY UPLC BEH C18分析柱分离,以10 mmol/L乙酸铵-0.2%甲酸水溶液和甲醇-乙腈(7:3, v/v)为流动相梯度洗脱,多反应监测(MRM)模式测定。24种染料的相关系数R2均大于0.99,回收率在61%~116%之间,方法的精密度(RSD, n=6)小于13%,定量限在0.1~4.0 μg/kg。该方法灵敏度高,操作简单、高效,适合于粮食及肉制品中24种禁用工业染料的同时定量及确证分析。
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| 关 键 词： | 超高效液相色谱-串联质谱 工业染料 固相萃取 粮食 肉制品 |
| 收稿时间： | 2013-03-07 |
Simultaneous determination of 24 industrial dyes in grain and meat products by ultra performance liquid chromatography-tandem mass spectrometry |
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| FENG Yuechao,JIA Li,HE Yahui,WANG Jianfeng,LIU Yan,FAN Xiaojing. Simultaneous determination of 24 industrial dyes in grain and meat products by ultra performance liquid chromatography-tandem mass spectrometry[J]. Chinese journal of chromatography, 2013, 31(10): 1021-1027. DOI: 10.3724/SP.J.1123.2013.03019 |
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| Authors: | FENG Yuechao JIA Li HE Yahui WANG Jianfeng LIU Yan FAN Xiaojing |
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| Affiliation: | Beijing Centre for Physical and Chemical Analysis, Beijing Engineering Research Center of Food Safety Analysis, Beijing 100089, China |
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| Abstract: | An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analytical method was established for the simultaneous determination of 24 forbidden industrial dyes in grain and meat products. The sample was extracted with methanol and acetonitrile, and cleaned-up by a WAX solid phase extraction column. The solution was separated on an ACQUITY UPLC BEH C18 column eluted with a mixture of 10 mmol/L ammonium acetate-0.2%formic acid aqueous solution and methanol-acetonitrile (7:3, v/v) as the mobile phases, and then analyzed in multiple reaction monitoring (MRM) mode. The correlation coefficients were above 0.99, the average recoveries were 61%-116%, and the relative standard deviations (RSD, n=6) were lower than 13%. The quantification limits were 0.1-4.0 μg/kg. This method is simple, effective, sensitive, and suitable for the determination and confirmation of the 24 forbidden industrial dyes in grain and meat products. |
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| Keywords: | ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) solid phase extraction (SPE) industrial dyes grain meat products |
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