| 高效液相色谱-线性指纹定量法评价人参归脾丸的三波长指纹图谱 |
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| 引用本文： | 孙国祥,豆小文,杨兰萍,刘中博. 高效液相色谱-线性指纹定量法评价人参归脾丸的三波长指纹图谱[J]. 色谱, 2013, 31(5): 456-461. DOI: 10.3724/SP.J.1123.2012.12004 |
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| 作者姓名： | 孙国祥 豆小文 杨兰萍 刘中博 |
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| 作者单位： | 沈阳药科大学药学院, 辽宁 沈阳 110016 |
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| 基金项目： | 国家自然科学基金重大研究计划课题(90612002). |
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| 摘 要： | 以样品指纹向量与对照指纹向量的多元化学组分间存在线性函数关系为基础,引入相关系数r、斜率a、截距b及线性定性相似度SL、线性定量相似度ML等概念,建立线性指纹定量法(linearly quantified fingerprint method, LQFM)模型以定性、定量控制中药质量。用高效液相色谱-二极管阵列检测法(HPLC-DAD)同时测定了10批人参归脾丸(Renshen Guipi Wan, RSGPW)分别在203、228和326 nm下的高效液相色谱指纹图谱,运用LQFM理论模型,鉴定RSGPW质量。LQFM能显著区别不同来源的产品,同一厂家产品质量控制一致。鉴定结果与已知的系统指纹定量法评价结果对比,LQFM对中药多元化学成分的复杂系统能实现客观的定性、定量评价。多波长指纹图谱能最大限度地兼顾中药各成分的紫外吸收特征,对中药质量控制具有更全面和可靠的特点。
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| 关 键 词： | 高效液相色谱 人参归脾丸 三波长指纹图谱 线性指纹定量法 中成药 |
| 收稿时间： | 2012-12-02 |
Evaluation of Renshen Guipi Wan quality by linearly quantified fingerprint method based on three-wavelength high performance liquid chromatographic fingerprints |
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| SUN Guoxiang,DOU Xiaowen,YANG Lanping,LIU Zhongbo. Evaluation of Renshen Guipi Wan quality by linearly quantified fingerprint method based on three-wavelength high performance liquid chromatographic fingerprints[J]. Chinese journal of chromatography, 2013, 31(5): 456-461. DOI: 10.3724/SP.J.1123.2012.12004 |
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| Authors: | SUN Guoxiang DOU Xiaowen YANG Lanping LIU Zhongbo |
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| Affiliation: | College of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China |
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| Abstract: | The theoretical model of linearly quantified fingerprint method (LQFM) for qualitative and quantitative control traditional Chinese medicine (TCM) has been developed based on the relationship of linear function between a sample and the reference fingerprint vector, as the concepts of correlation coefficient r, slope a, intercept b, linearly qualitative similarity SL, linearly quantitative similarity ML and so on were introduced into the study. The high performance liquid chromatography coupled with diode array detector (DAD) was used to simultaneously obtain chromatograms of ten batches of Renshen Guipi Wan (RSGPW) at 203, 228 and 326 nm, separately. The model of LQFM was applied to evaluate the three-wavelength fingerprints for the quality control of RSGPW, it could obviously distinguish RSGPW from different resources and the results showed the quality was almost consistent from the same manufacture. The results of LQFM were compared with those of the systematically quantified fingerprint method (SQFM), a known method for quality control of TCM. It proved that LQFM can evaluate qualitatively and quantitatively the complex system of TCM which is composed of diverse chemical components. The quality control can be better and more reliable by multiple-wavelength fingerprints which give full consideration of the ultraviolet absorption characteristics of different components. |
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| Keywords: | high performance liquid chromatography (HPLC) linearly quantified fingerprint method (LQFM) three-wavelength fingerprints (THW-FPs) Renshen Guipi Wan (RSGPW) traditional Chinese medicine (TCM) |
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