Electrochemical Determination of Homocysteine at Disposable Graphite Electrodes |
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| Authors: | Ece Eksin Arzum Erdem |
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| Affiliation: | 1. Ege University, Faculty of Pharmacy, Analytical Chemistry Department, 35100, Bornova, Izmir, Turkey;2. Ege University, The Institute of?Natural and Applied Sciences, Biotechnology Department, 35100, Bornova, Izmir, Turkey |
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| Abstract: | In the case of disruption of Hcy metabolism, the blood level of Hcy increases and it causes particularly the cardiovascular diseases, cancer, dementia and Parkinson’s disease. Thus, the sensitive analysis of Hcy levels in biological fluids is very important. Hcy analysis was performed herein using very practical and cost‐effective protocol using differential pulse voltammetry and graphite electrode. Detection limit of Hcy was found to be 1.21 µM in the linear range from 2 µM to 20 µM. The electrochemical Hcy detection in artificial urine medium was also successfully performed even in the presence of L ‐Cysteine, L ‐Methionine and Glutathione. |
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| Keywords: | Homocysteine Direct electrochemical detection Protein Urine Pencil graphite electrode |
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