A Highly K+‐Selective Two‐Photon Fluorescent Probe |
| |
| Authors: | Dr. Thomas Schwarze Janine Riemer Dr. Sascha Eidner Prof. Hans‐Jürgen Holdt |
| |
| Affiliation: | 1. Institut für Chemie, Anorganische Chemie, Universit?t Potsdam, Karl‐Liebknecht‐Str. 24–25, 14476 Golm (Germany);2. Institut für Chemie, Physikalische Chemie, Universit?t Potsdam, Karl‐Liebknecht‐Str. 24–25, 14476 Golm (Germany) |
| |
| Abstract: | A highly K+‐selective two‐photon fluorescent probe for the in vitro monitoring of physiological K+ levels in the range of 1–100 mM is reported. The two‐photon excited fluorescence (TPEF) probe shows a fluorescence enhancement (FE) by a factor of about three in the presence of 160 mM K+, independently of one‐photon (OP, 430 nm) or two‐photon (TP, 860 nm) excitation and comparable K+‐induced FEs in the presence of competitive Na+ ions. The estimated dissociation constant (Kd) values in Na+‐free solutions (KdOP=(28±5) mM and KdTP=(36±6) mM ) and in combined K+/Na+ solutions (KdOP=(38±8) mM and KdTP=(46±25) mM ) reflecting the high K+/Na+ selectivity of the fluorescent probe. The TP absorption cross‐section (σ2PA) of the TPEF probe+160 mM K+ is 26 GM at 860 nm. Therefore, the TPEF probe is a suitable tool for the in vitro determination of K+. |
| |
| Keywords: | click chemistry fluorescence fluorescent probes potassium two‐photon |
|
|
