A simple high‐performance liquid chromatography for the determination of linezolid in human plasma and saliva |
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| Authors: | Shuuji Hara Masanobu Uchiyama Masami Yoshinari Taichi Matsumoto Shiro Jimi Atsushi Togawa Tohru Takata Yasushi Takamatsu |
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| Affiliation: | 1. Faculty of Pharmaceutical Sciences Fukuoka University, Fukuoka, Japan;2. Department of Pharmacy, Fukuoka University Hospital, Fukuoka, Japan;3. Central Laboratory for Pathology and Morphology, Department of Medicine, Fukuoka University, Fukuoka, Japan;4. Division of Medical Oncology, Hematology and Infectious Diseases, Department of Medicine, Fukuoka University, Fukuoka, Japan |
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| Abstract: | Linezolid is an antimicrobial agent for the treatment of multiresistant Gram‐positive infections. A practical high‐performance liquid chromatography method was developed for the determination of linezolid in human plasma and saliva. Linezolid and an internal standard (o‐ethoxybenzamide) were extracted from plasma and saliva with ethyl acetate and analyzed on a Capcell Pak C18 MG column with UV detection at 254 nm. The calibration curve was linear through the range 0.5–50 µg/mL using a 200 μL sample volume. The intra‐ and interday precisions were all <6.44% for plasma and 5.60% for saliva. The accuracies ranged from 98.8 to 110% for both matrices. The mean recoveries of linezolid were 80.8% for plasma and 79.0% for saliva. This method was used to determine the plasma and saliva concentrations of linezolid in healthy volunteers who were orally administered a 600 mg dose of linezolid. Our liquid–liquid extraction procedure is easy and requires a small volume of plasma or saliva (200 μL). This small volume can be advantageous in clinical pharmacokinetic studies, especially if children participate. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | linezolid HPLC-UV plasma saliva therapeutic drug monitoring |
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