Comparison among three anion exchange chromatographic supports to capture erythropoietin from cell culture supernatant Lourdes |
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| Authors: | Lourdes HERN&# NDEZ Diobel STEWART Lourdes ZUMALAC&# RREGUI Daniel AMARO |
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| Affiliation: | 1. Development Product Department, Manufacturing Plant of Recombinant Proteins and Vaccines, Center for Molecular Immunology, Havana 11600, Cuba;2. Department of Chemical Engineering, Faculty of Chemical Engineering, Higher Polytechnic Institute José Antonio Echeverría, Havana 19390, Cuba |
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| Abstract: | Affinity and ion exchange conventional chromatography have been used to capture erythropoietin (EPO) from mammalian cell culture supernatant. Currently, chromatographic adsorbent perfusion is available, however a limited number of applications have been found in the literature. In this work, three anion exchange chromatographic supports (gel, membrane and monolithic) were evaluated in the capture step of the recombinant erythropoietin purification process. The influences of load and flow rate on each support performance were analyzed. Also the purity of the EPO molecules was determined. A productivity analysis, as a decision tool for larger scale implementation, was done. As a conclusion, the evaluated supports are technically suitable to capture EPO with adequate recovery and good purity. However, the monolithic column admits high operating velocity, showing the highest adsorption capacity and productivity. |
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| Keywords: | anion exchange chromatography purification recombinant erythropoietin supports |
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