Red–Green–Blue Trichromophoric Nanoparticles with Dual Fluorescence Resonance Energy Transfer: Highly Sensitive Fluorogenic Response Toward Polyanions |
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| Authors: | Jinjia Xu Dr. Atsuro Takai Prof. Dr. Masayuki Takeuchi |
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| Affiliation: | 1. Molecular Design & Function Group, National Institute for Materials Science (NIMS), Tsukuba, Ibaraki, Japan;2. Department of Materials Science and Engineering, Graduate School of Pure and Applied Science, University of Tsukuba, Tsukuba, Ibaraki, Japan |
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| Abstract: | A red–green–blue (RGB) trichromophoric fluorescent organic nanoparticle exhibiting multi‐colour emission was constructed; the blue‐emitting cationic oligofluorene nanoparticle acted as an energy‐donor scaffold to undergo fluorescence resonance energy transfer (FRET) to a red‐emitting dye embedded in the nanoparticle (interior FRET) and to a green‐emitting dye adsorbed on the surface through electrostatic interactions (exterior FRET). Each FRET event occurs independently and is free from sequential FRET, thus the resultant dual‐FRET system exhibits multi‐colour emission, including white, in aqueous solution and film state. A characteristic white‐emissive nanoparticle showed visible responses upon perturbation of the exterior FRET efficiency by acceptor displacement, leading to highly sensitive responses toward polyanions in a ratiometric manner. Specifically, our system exhibits high sensitivity toward heparin with an extremely low detection limit. |
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| Keywords: | biosensors FRET multi-colour emission nanoparticles supramolecular assembly |
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