| 阵列式对电极介电电泳芯片及其用于细胞分离富集研究 |
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| 引用本文： | 徐溢,曹强,曾雪,吴永杰,张文品. 阵列式对电极介电电泳芯片及其用于细胞分离富集研究[J]. 高等学校化学学报, 2009, 30(5): 876-881 |
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| 作者姓名： | 徐溢 曹强 曾雪 吴永杰 张文品 |
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| 作者单位： | 新型微纳器件与系统技术国防重点学科实验室, 微纳系统及新材料技术国际研发中心, 重庆大学化学化工学院, 重庆 400030 |
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| 基金项目： | 国家自然科学基金，国家高技术研究发展计划(863计划)，国际科技合作项目，重庆市自然科学基金重点项目，重庆大学研究生科技创新项目 |
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| 摘 要： | 基于介电电泳原理, 设计并制作了一种新型的能够用于细胞分离和富集的微流控介电电泳芯片. 该芯片由沉积有金电极的石英基片和带有微管道的聚二甲基硅氧烷(PDMS)盖片组成. 通过在管道底部布置间距不同的对电极阵列, 增大了正介电电泳力在管道中的有效作用范围, 能够在降低施加电压的同时, 实现对流动体系中细胞样品的捕获. 在3 V和3 MHz条件下, 该DEP芯片对人血红细胞的捕获效率达到83%; 进一步通过将肝癌细胞捕获在芯片电极上可实现对红细胞和肝癌细胞混合样品的分离, 在5 V和400 kHz条件下对肝癌细胞的捕获效率达到86%.
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| 关 键 词： | 阵列式对电极 介电电泳芯片 细胞分离与富集 |
| 收稿时间： | 2009-01-12 |
Research of Cell Concentration and Separation on the Dielectrophoretic Chip with Arrayed Opposite Electrodes |
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| XU Yi,CAO Qiang,ZENG Xue,WU Yong-Jie,ZHANG Wen-Pin. Research of Cell Concentration and Separation on the Dielectrophoretic Chip with Arrayed Opposite Electrodes[J]. Chemical Research In Chinese Universities, 2009, 30(5): 876-881 |
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| Authors: | XU Yi CAO Qiang ZENG Xue WU Yong-Jie ZHANG Wen-Pin |
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| Affiliation: | Key Disciplines Lab of Novel Micro-nano Devices and System Technology, International R & D Center of Micro-nano Systems and New Materials Technology, Chemistry and Chemical Engineering College, Chongqing University, Chongqing 400030, China |
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| Abstract: | A prototype microfluidic dielectrophoretic(DEP) chip for cell separation and concentration based on principle of DEP was presented in this paper.The proposed microfluidic DEP chip was bonded directly with glass substrate deposited Au micro electrodes and polydimethylsiloxane(PDMS) cover plate with designed micro-channels.The effective area of positive DEP was increased by the designed electrode array which composed of several groups of opposite electrodes with different distances on the bottom of glass chip... |
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| Keywords: | Arrayed opposite electrodes Microfluidic DEP chip Separation and concentration of cells |
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