| PS胶体粒子表面逐层自组装固定化SOD及其生物活性 |
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| 引用本文： | 何成毅,梁振鹏,王朝阳,刘新星,童真. PS胶体粒子表面逐层自组装固定化SOD及其生物活性[J]. 高等学校化学学报, 2005, 26(1): 88-92 |
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| 作者姓名： | 何成毅 梁振鹏 王朝阳 刘新星 童真 |
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| 作者单位： | 华南理工大学材料科学研究所, 广州 510640 |
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| 基金项目： | 国家自然科学基金;广东省自然科学基金;广东省广州市科技攻关项目 |
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| 摘 要： | 通过逐层自组装技术成功地把超氧化物歧化酶(SOD)吸附在聚苯乙烯(PS)胶体粒子表面.zeta电位和TEM证明了聚阳离子或聚阴离子型SOD与相反电荷的聚电解质在PS胶体粒子表面的交替吸附.通过测定SOD被胶体粒子吸附后上清液的生物活性,得到聚阴离子型SOD(pH=8.0)和聚阳离子型SOD(pH=4.3)在PS胶体粒子表面的吸附量分别为12和51IU,相对活性分别为23.4%和2.9%.聚阴离子型SOD在PS胶体粒子表面能形成平滑规整的膜,导致较高的相对活性.研究结果表明,通过调节pH值,可以优化自组装固定化酶的聚集状态和生物活性
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| 关 键 词： | 超氧化物歧化酶 逐层自组装 固定化酶 聚电解质多层膜 PS胶体粒子 |
| 文章编号： | 0251-0790(2005)01-0088-05 |
| 收稿时间： | 2004-07-30 |
Immobilization of Superoxide Dismutase by Layer-by-layer Assembly on Surface of PS Colloid Particles and Their Bioactivity |
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| HE Cheng-Yi,LIANG Zhen-Peng,WANG Chao-Yang,LIU Xin-Xing,TONG Zhen. Immobilization of Superoxide Dismutase by Layer-by-layer Assembly on Surface of PS Colloid Particles and Their Bioactivity[J]. Chemical Research In Chinese Universities, 2005, 26(1): 88-92 |
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| Authors: | HE Cheng-Yi LIANG Zhen-Peng WANG Chao-Yang LIU Xin-Xing TONG Zhen |
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| Affiliation: | Research Institute of Materials Science, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | Novel enzyme multilayer films on the surface of polystyrene(PS) colloid particles was fabricated by layer-by-layer(LbL) self-assembly. Superoxide dismutase(SOD) was adsorbed on the PS particles as either polycation or polyanion switched by adjusting pH alternatingly with the oppositely charged polyelectrolytes. zeta-Potential and TEM results indicated the regular and stepwise growth of the multilayer structure. The amount of the immobilized SOD was estimated from the difference in SOD bioactivity of the supernatant after adsorption and SOD solution before adsorption by using the pyrogallol oxidation method. The immobilization amount of SOD was 12 and 51 IU when adsorbed in pH=8.0 as a polyanion and in pH=4.3 as a polycation, respectively. However, the relative activity of the former was 23.4% while that of the latter was 2.9%, compared to that of free SOD in aqueous solution. Anionic SOD was found to form more regular and smooth layers on the PS particle surface and cationic SOD to aggregate. By adjusting pH of the adsorption solution we can optimize the assembled status and bioactivity of particle-immobilized enzyme. |
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| Keywords: | Superoxide dismutase(SOD) Layer-by-layer self-assembly Immobilized enzyme Polyelectrolyte multilayer films PS colloid particle |
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