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| 毛细管电泳-间接激光光热干涉检测方法研究 | |
| 引用本文： | 任吉存,李斌成,邓延倬,程介克. 毛细管电泳-间接激光光热干涉检测方法研究[J]. 高等学校化学学报, 1995, 16(8): 1182 |
| 作者姓名： | 任吉存 李斌成 邓延倬 程介克 |
| 作者单位： | 1. 武汉大学化学系, 武汉, 430072;2. 武汉大学分析测试科学系武汉, 430072 |
| 摘 要： | 本文提出毛细管电泳-间接激光光热干涉检测新方法。采用吸收系数大、吸收波长与泵浦激光(He-Ne)波长匹配性能较好的亚甲蓝溶液为背景电解质溶液,加入乙醇减少了毛细管壁对亚甲蓝的吸附作用。并将间接光热干涉检测法用于氨基酸毛细管电泳分离检测,对赖氨酸检测限达5×10-6mol/L(S/N=2). |
| 关 键 词： | 毛细管电泳 间接光热检测 氨基酸 分离 |
| 收稿时间： | 1994-10-12 |
Studies on Indirect Thermo-Optical Detection for Capillary Electrophoresis |
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| REN Ji-Cun,LI Bin-Cheng,DENG Yan-Zhuo, CHENG Jie-Ke. Studies on Indirect Thermo-Optical Detection for Capillary Electrophoresis[J]. Chemical Research In Chinese Universities, 1995, 16(8): 1182 | |
| Authors: | REN Ji-Cun LI Bin-Cheng DENG Yan-Zhuo CHENG Jie-Ke |
| Affiliation: | 1. Department of Chemistry, Wuhan University, Wuhan, 430072;2. Department of Analysis and Testing Science, Wuhan University, Wuhan, 430072 |
| Abstract: | Indirect thermo-optical detection for capillary electrophoresis was presented first by combining indirect detection with thermo-optical method.In this detection,methylene blue solution was used as background electrolyte solution,because it has large molar absorption coefficient at 633 nm. This wavelength matches well with that of He-Ne laser(632.8 nm).The detection was applied to detect amino acids in capillary zone electrophoresis separation. The detection limit for lysine was 5×10-6mol/L(S/N=2). |
| Keywords: | Capillary electrophoresis Indirect thermo-optical detection Amino acid Separation |
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