Simultaneous determination of 7‐O‐succinyl macrolactin A and its active major metabolite,macrolactin A in dog plasma using high‐performance liquid chromatography with UV detection |
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| Authors: | Eunyoung Kim Beomsoo Shin Kwang‐il Kwon Joon Seok Bang Wonku Kang |
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| Affiliation: | 1. College of Pharmacy, Chung‐Ang University, Seoul, South Korea;2. College of Pharmacy, Catholic University of Daegu, Kyoungbuk, South Korea;3. College of Pharmacy, Chungnam National University, Daejeon, South Korea;4. Graduate School of Clinical Pharmacy, Sookmyung Women's University, Seoul, South Korea |
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| Abstract: | We developed a method for the simultaneous quantification of 7‐O‐succinyl macrolactin A and its active metabolite, macrolactin A, in dog plasma. After protein precipitation with acetonitrile including flufenamic acid as an internal standard, 7‐O‐succinyl macrolactin A, macrolactin A, and flufenamic acid were chromatographed on a reverse‐phase C18 analytical column. The mobile phase, consisting of 20 mM acetate buffer and acetonitrile, was eluted using a gradient program at 1 mL/min, and the UV absorbance was measured at 230 nm. The retention times of 7‐O‐succinyl macrolactin A, flufenamic acid, and macrolactin A were 3.4, 4.8, and 6.9 min, respectively. The coefficient of variation in the assay precision for both substances was less than 6%, and the accuracy ranged from 96 to 105%. This method was used to measure the concentrations of 7‐O‐succinyl macrolactin A and macrolactin A in dog plasma following an intravenous administration of a single dose (25 mg/kg) of 7‐O‐succinyl macrolactin A salt. |
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| Keywords: | Dog plasma High‐performance liquid chromatography Macrolactin A 7‐O‐Succinyl macrolactin A UV detection |
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