Inverting the G‐Tetrad Polarity of a G‐Quadruplex by Using Xanthine and 8‐Oxoguanine |
| |
| Authors: | Dr. Vee Vee Cheong Dr. Christopher Jacques Lech Dr. Brahim Heddi Prof. Dr. Anh Tuân Phan |
| |
| Affiliation: | School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371 (Singapore) |
| |
| Abstract: | G‐quadruplexes are four‐stranded nucleic acid structures that are built from consecutively stacked guanine tetrad (G‐tetrad) assemblies. The simultaneous incorporation of two guanine base lesions, xanthine (X) and 8‐oxoguanine (O), within a single G‐tetrad of a G‐quadruplex was recently shown to lead to the formation of a stable G?G?X?O tetrad. Herein, a judicious introduction of X and O into a human telomeric G‐quadruplex‐forming sequence is shown to reverse the hydrogen‐bond polarity of the modified G‐tetrad while preserving the original folding topology. The control exerted over G‐tetrad polarity by joint X?O modification will be valuable for the design and programming of G‐quadruplex structures and their properties. |
| |
| Keywords: | DNA structures G‐quadruplexes NMR spectroscopy 8‐oxoguanine xanthine |
|
