Design of a Hyperpolarized Molecular Probe for Detection of Aminopeptidase N Activity |
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| Authors: | Ryunosuke Hata Dr. Hiroshi Nonaka Dr. Yoichi Takakusagi Prof. Dr. Kazuhiro Ichikawa Prof. Dr. Shinsuke Sando |
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| Affiliation: | 1. Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan;2. Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan;3. Incubation Center for Advanced Medical Science, Kyushu University, Fukuoka, Japan;4. Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka, Japan |
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| Abstract: | Aminopeptidase N (APN) is an important enzyme that is involved in tumor angiogenesis. Detection of APN activity can thus lead to early diagnosis and elucidation of tumor development. Although some molecular probes for APN have been developed, the detection of APN activity in opaque biological samples remains a challenge. To this end, we designed a hyperpolarized NMR probe [1‐13C]Ala‐NH2 which satisfies the prerequisites for APN detection, namely, sufficient retention of the hyperpolarized state, a high reactivity to APN, and an APN‐induced chemical shift change. The [1‐13C]Ala‐NH2 probe allowed sensitive detection of APN activity using 13C NMR spectroscopy. |
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| Keywords: | aminopeptidases biosensors dynamic nuclear polarization hyperpolarization NMR spectroscopy |
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