| 高效毛细管电泳-电化学检测对多羟基抗生素分离测定的研究 |
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| 引用本文： | 方禹之,方晓明,叶建农. 高效毛细管电泳-电化学检测对多羟基抗生素分离测定的研究[J]. 高等学校化学学报, 1995, 16(10): 1514 |
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| 作者姓名： | 方禹之 方晓明 叶建农 |
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| 作者单位： | 华东师范大学化学系, 上海, 200062 |
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| 基金项目： | 中国科学院长春应用化学研究所电分析化学开放实验室 |
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| 摘 要： | 用自制的高效毛细管电泳-电化学检测装置,以350μm铜圆盘电极为工作电极,在碱性溶液中,对7种多羟基抗生素的分离进行了研究。结果表明:浓度线性范围可达2至3个数量级,最低检测限为5.0×10-7mol/L.在8h内连续测定,峰电流和迁移时间的相对标准偏差分别为7.6%和2.2%.实际样品(丁胺卡那霉素)测定回收率为99.6%.
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| 关 键 词： | 毛细管电泳 电化学检测 多羟基抗生素 铜电极 |
| 收稿时间： | 1995-04-10 |
Determination of Polyhydroxy Antibiotics by Capillary Electrophoresis with Amperometric Detection at a Copper Electrode |
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| FANG Yu-Zhi, FANG Xiao-Ming, YE Jian-Nong. Determination of Polyhydroxy Antibiotics by Capillary Electrophoresis with Amperometric Detection at a Copper Electrode[J]. Chemical Research In Chinese Universities, 1995, 16(10): 1514 |
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| Authors: | FANG Yu-Zhi FANG Xiao-Ming YE Jian-Nong |
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| Affiliation: | Department of Chemistry, East China Normal University, Shanghai, 200062 |
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| Abstract: | Capillary electrophoresis(CE) was employed to separate polyhydroxy antibiotics in alkaline solution, combined with electrochemical detection at copper electrode. The electrode used was a 350 μm diameter Cu disk operated in a wall-jet configuration. Asample mixture containing 7 different polyhydroxy antibibeics was separated within 30min. The linearity varied over a 2-3 order of magnitude and the detection limits were 0.5 μmol/L.The reproducibility for the peak current and the migration time of 50 μ mol/Ldoublemycin injected every hour in a period of 8h, was 7.6% and 2.2% RSD, respectively, and the recovery of the determination of the real sample was 99.6%. |
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| Keywords: | Capillary electrophoresis Electrochemical detection Polyhydroxy antibiotics Copper electrode |
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