Hydrophilic interaction chromatography with Fourier transform mass spectrometry of monosaccharide anhydrides |
| |
| Authors: | David Matějíček Jana Vašíčková |
| |
| Affiliation: | 1. N.T.V.S.R, Brno, Czech Republic;2. Holaja, Brno, Czech Republic |
| |
| Abstract: | A fast, selective, and sensitive method for the determination of three monosaccharide anhydrides (galactosan, mannosan, levoglucosan), based on hydrophilic interaction chromatography and Fourier transform mass spectrometry, was successfully developed. The simple experimental stationary phase and mass spectrometry performance screening allowed the selection of the best available chromatographic and mass spectrometry conditions. Thus, the chromatographic separation was performed on a highly selective stationary phase containing a zwitterionic phosphorylcholine group and the monosaccharide anhydrides were detected as [M+HCOO]? adduct in the negative mode. The method showed accuracy in the range of 84–111 and 89–102% with interbatch precision expressed as relative standard deviations of 5.6–15.4 and 5.0–9.0% for the aerosol extract and snow samples, respectively. The limit of quantification in absolute values ranged from 10 to 30 pg, the limit of quantification, expressed as concentration, ranged was 0.3–0.9 ng/m3 for aerosol and 10–20 ng/mL for snow samples. The method was successfully applied for the determination of monosaccharide anhydrides in aerosol and snow samples. |
| |
| Keywords: | Fourier transform mass spectrometry Hydrophilic interaction chromatography Monosaccharide anhydrides |
|
|
