Cell‐Penetrating Poly(disulfide) Assisted Intracellular Delivery of Mesoporous Silica Nanoparticles for Inhibition of miR‐21 Function and Detection of Subsequent Therapeutic Effects |
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| Authors: | Dr. Changmin Yu Linghui Qian Dr. Jingyan Ge Jiaqi Fu Dr. Peiyan Yuan Samantha C. L. Yao Prof. Dr. Shao Q. Yao |
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| Affiliation: | 1. Department of Chemistry, National University of Singapore, Singapore, Singapore;2. College of Materials Science & Engineering, South China University of Technology, Guangzhou, China;3. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou, China |
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| Abstract: | The design of drug delivery systems capable of minimal endolysosomal trapping, controlled drug release, and real‐time monitoring of drug effect is highly desirable for personalized medicine. Herein, by using mesoporous silica nanoparticles (MSNs) coated with cell‐penetrating poly(disulfide)s and a fluorogenic apoptosis‐detecting peptide (DEVD‐AAN), we have developed a platform that could be uptaken rapidly by mammalian cells via endocytosis‐independent pathways. Subsequent loading of these MSNs with small molecule inhibitors and antisense oligonucleotides resulted in intracellular release of these drugs, leading to combination inhibition of endogenous miR‐21 activities which was immediately detectable by the MSN surface‐coated peptide using two‐photon fluorescence microscopy. |
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| Keywords: | cell-penetrating poly(disulfide) drug delivery endocytosis nanoparticles small-molecule inhibitor |
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